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Sau DNA Polymerase
Sau DNA polymerase is derived from the DNA polymerase (Sau) I gene of Staphylococcus aureus, which is obtained by cutting 1-293 AA at its N-terminal. This enzyme retains the 5'- 3' polymerase activity of DNA polymerase but lacks 5' - 3' and 3' - 5' exonuclease activities, which can be used for recombinase amplification.
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Cat. No.: SAU-500 (for 500U)
Cat. No.: SAU-2500 (for 2,500U)
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
Description
Sau DNA polymerase is a DNA polymerase with strand displacement activity, which can be used for recombinase isothermal amplification. The protein-DNA complex formed by the combination of recombinant enzyme UvsX and primers can find homologous sequences in double-stranded DNA. Once the primer locates the homologous sequence, a strand displacement reaction will occur. Sau DNA polymerase starts DNA synthesis, and exponentially amplifies the target region on the template. The replaced DNA strand will bind to SSB to prevent further replacement.
Applications
- Primer labeling
- Second strand cDNA synthesis
- DNA strand displacement synthesis
- Single dA tailing
Heat Inactivation
75°C, 20 min.
Unit Definition
One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.
Storage
The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.
Reagents for Recombinase Polymerase Amplification (RPA)
- T4 UvsX Recombinase
- T4 UvsY Protein
- T4 Gene 32 Protein
- Bsu DNA Polymerase
- Sau DNA Polymerase
- Exonuclease III
- Bst DNA/RNA Polymerase
- Endonuclease IV
Instruction: RPA Reaction System (for reference only)
RPA Isothermal Amplification Kits
In addition to the RPA raw material enzymes mentioned earlier, we offer a diverse selection of kits designed to meet your specific needs. Explore our exceptional offerings:
- RPA DNA Amplification Reagent Kit (Basic Type)
- RPA DNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
- RPA RNA Amplification Reagent Kit (Basic Type)
- RPA RNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
- RPA DNA Rapid Amplification Reagent Kit (ExolII Fluorescence Enhancement Type)
- RPA RNA Rapid Amplification Reagent Kit (ExollI Fluorescence Enhancement Type)
- Influenza A Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Influenza B Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification Fluorescence Method)
- 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification CRISPR Method)
- African Swine Fever Virus Nucleic Acid Detection Kit (Isothermal Amplification CRISPR Method)
- White Spot Syndrome Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Monkeypox Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Adenovirus Type 3 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Adenovirus Type 7 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Streptococcus Pyogenes Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
