Sau DNA polymerase is derived from the DNA polymerase (Sau) I gene of Staphylococcus aureus, which is obtained by cutting 1-293 AA at its N-terminal. This enzyme retains the 5'- 3' polymerase activity of DNA polymerase but lacks 5' - 3' and 3' - 5' exonuclease activities, which can be used for recombinase amplification.
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Cat. No.: SAU-500 (for 500U)
Cat. No.: SAU-2500 (for 2,500U)
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
Sau DNA polymerase is a DNA polymerase with strand displacement activity, which can be used for recombinase isothermal amplification. The protein-DNA complex formed by the combination of recombinant enzyme UvsX and primers can find homologous sequences in double-stranded DNA. Once the primer locates the homologous sequence, a strand displacement reaction will occur. Sau DNA polymerase starts DNA synthesis, and exponentially amplifies the target region on the template. The replaced DNA strand will bind to SSB to prevent further replacement.
Second strand cDNA synthesis
DNA strand displacement synthesis
Single dA tailing
75°C, 20 min.
One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.
The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.
Reagents for Recombinase Polymerase Amplification (RPA)
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