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T4 UvsX Recombinase

T4 UvsX Recombinase

T4 UvsX recombinase, derived from T4 phage, is a homolog of the RecA/Rad51 family. RecA/Rad51 recombinase family plays an important role in the repair of double-stranded DNA break and the restart of replication forks. T4 UvsX recombinase can anchor on single-stranded DNA together with other recombinase and activate it to scan for homologous sequences on other double-stranded DNA for further strand replacement. The enzyme has no nuclease activity.
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Cat. No.: UVSX-300 (for 300 μg)

Cat. No.: UVSX-3k (for 3 mg)

 

Component

  • T4 UvsX Recombinase (3 μg/μl, glycerol free): 100 μl
Optional (available by inquiry)
  • 2xRPA Buffer Mix: 0.5 ml
  • 280mM Mg(OAC)2: 0.1 ml

 

Application

  • DNA isothermal amplification
  • RPA amplification
  • Genetic testing

 

Heat Inactivation

60°C, 10min.

 

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.

 

Storage

The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.

 

Reagents for Recombinase Polymerase Amplification (RPA)

  • T4 UvsX Recombinase
  • T4 UvsY Protein
  • T4 Gene 32 Protein
  • Bsu DNA Polymerase
  • Sau DNA Polymerase
  • Exonuclease III
  • Bst DNA/RNA Polymerase
  • Endonuclease IV

 

Instruction: RPA Reaction System (for reference only)

 

RPA Isothermal Amplification Kits

In addition to the RPA raw material enzymes mentioned earlier, we offer a diverse selection of kits designed to meet your specific needs. Explore our exceptional offerings:

  • RPA DNA Amplification Reagent Kit (Basic Type)
  • RPA DNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
  • RPA RNA Amplification Reagent Kit (Basic Type)
  • RPA RNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
  • RPA DNA Rapid Amplification Reagent Kit (ExolII Fluorescence Enhancement Type)
  • RPA RNA Rapid Amplification Reagent Kit (ExollI Fluorescence Enhancement Type)
  • Influenza A Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • Influenza B Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification Fluorescence Method)
  • 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification CRISPR Method)
  • African Swine Fever Virus Nucleic Acid Detection Kit (Isothermal Amplification CRISPR Method)
  • White Spot Syndrome Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • Monkeypox Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • Adenovirus Type 3 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • Adenovirus Type 7 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
  • Streptococcus Pyogenes Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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