Bsu DNA Polymerase, derived from Bacillus subtilis, was obtained by truncating the first 296 AAs of the Bacillus subtilis DNA polymerase (Bsu) I gene. This polymerase retains its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. Bsu DNA Polymerase naturally lacks the 3’→ 5’ exonuclease activity, which can be used for recombinase amplification.
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Cat. No.: BSU-2k (for 2000U)
Cat. No.: BSU-10k (for 10000U)
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
Second strand cDNA synthesis
DNA strand displacement synthesis
Single dA tailing
75°C, 20 min.
One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.
The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.
Due to the lack of the 3’→ 5’ exonuclease activity, Bsu DNA Polymerase (Large Fragment) cannot excise 3' protruding ends and is therefore not suitable for generating blunt ends.
At 25°C, Bsu DNA Polymerase (Large Fragment) retains 50% activity, which is twice as high as the Klenow fragment (3´→5´ exo-) at the same temperature.
Reagents for Recombinase Polymerase Amplification (RPA)
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