DNA-RNA Conversion
Transcription of DNA to RNA
A strand of DNA is used as a template for synthesizing a strand of RNA by RNA polymerase (e.g. T7 Thermostable RNA Polymerase). During this process, RNA polymerase binds to a site called the promoter and starts copying the nucleotides, where deoxyribonucleotides are replaced by ribonucleotides.
Reverse Transcription of RNA to DNA
Complementary DNA (cDNA) is generated from an RNA template by reverse transcriptase (e.g. Ultra Thermo-stable M-MLV Reverse Transcriptase). During this process, RNasin is usually used to prevent the degradation of the RNA templates.
mRNA Synthesis
Offering high-quality building blocks for mRNA synthesis
High-quality Building Blocks
With the rapid development of biotech, the mRNA vaccine prepared by RNA in vitro synthesis has a great potential for the prevention of many diseases such as tumors, infectious diseases, and chronic diseases. At SBS Genetech, we are at the forefront of offering high-quality building blocks for mRNA synthesis. Our building blocks are free of RNase and DNase and are suitable for any molecular biology application that requires pure ribonucleotides.
Backbone: NTPs
100mM Ribonucleotide Triphosphate (NTP or rNTP) is a ready-to-use solution of NTP (UTP, CTP, GTP, or ATP) at a concentration of 100mM, whose purity is up to 99% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure ribonucleotides.
