Custom Oligo Pools
Description
We are leading the new trend in oligo pool synthesis technology, having independently developed the second-generation high-throughput synthesis technology using inkjet printing. This technology boasts a throughput of 12K-8M and synthesis lengths of up to 170nt, offering customers a wider range of options. In contrast, traditional column-based first-generation low-throughput synthesis technology offers only 96, 192, or 768 throughput options, with synthesis lengths limited to 120nt.
Features
- There are 12,472, 91,776, or 8,000,000 oligos in each pool, that is, 12,472, 91,776, or 8,000,000 primers can be provided at one time.
- The error rate is only 0.5%, that is, only one error base in 200 bases is found.
- Each primer has a full-length DNA fragment of 1 fmole.
- The oligos up to 170 bases can be synthesized.
- Can be used in synthetic biology, gene synthesis, targeted sequencing, and complex DNA library.
12K Oligo Pools (Up to 12,472 Oligos)
- 2-3 weeks turnaround time.
92K Oligo Pools (Up to 91,776 Oligos)
- 2-3 weeks turnaround time.
8M Oligo Pools (Up to 8,000,000 Oligos) are also available by inquiry.
Oligo Pools Application Guide
We can provide some common guidelines for primer design and amplification of oligo pools. But for specific experiments, our guidelines can only be used as general guidelines. You need to adjust the specific experimental steps according to the conditions. The guiding principles for the use of oligo pools mainly include the following points:
- A set of universal primers will be used before amplification. PCR amplification can achieve the effect of purifying oligo pools because the failed primer sequence (the primer sequence missing at 5 'end) cannot be amplified.
- To achieve a higher annealing temperature, we suggest using a high Tm value or longer primers. Higher annealing temperatures can reduce primer mismatch and enhance the specificity of amplification.
- In PCR amplification, we recommend not using conventional Taq polymerase, but using hot start enzymes, such as Fusion, Q5, Pfu, Vent, Platinum Taq Polymerase, and PrimeTaq™ HotStart Direct PCR DNA Polymerase. The selection of enzymes should be cautious, and some enzymes need to be tested before use.
- Due to the different sequence lengths, primer designs, and polymerase systems of different users, the usage of starting templates is also different. For PCR templates, it is suggested to use 1/100, 1/400, 1/1600, 1/6400, and 1/25600 of the total reaction volume for the gradient test. Set the number of PCR cycles, such as 25 cycles to amplify, and use gel or Bioanalyzer to detect the amount of PCR products. As long as the quantity of PCR products meets the requirements, the smaller amount should be chosen as the final amount of the template.
- If multiple sequences need to be amplified from the product, nested PCR is used to amplify all the sequences, and then internal primers are used to amplify each group of sequences. Only one round of PCR may not be enough for getting a good result.
- The basic strategy of primer removal depends on the specific needs of the downstream application of oligo pools, such as blunt end or sticky end.
- For normal cloning, users can insert the restriction sites recognized by type IIS restriction enzymes into the universal primers and then remove the universal primers after PCR amplification. These restriction enzymes can digest near the recognition site to maintain the integrity of variable sequences. After the digestion, the oligo sequences can be inserted into the vector.
SBS Genetech is recognized as one of the global major leading industry players in Oligonucleotide Synthesis by third-party market researchers. For more details, please visit Oligonucleotide Synthesis Services Market landscape, Top Competitor Analysis, Revenue, Sales With Forecast Data from 2022 to 2028.Selected Published Papers
SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood
Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level
Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100
All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).
Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant
Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828
To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)
A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes
Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543
HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier
Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation
Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055
Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).
Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection
Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089
Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......
Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor
Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019
Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC
Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions
Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014
Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).
A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin
International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211
The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.
Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer
Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465
The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.
Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva
Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015
The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...
Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection
Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318
Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
