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Cat. No.: TSDSAP-1k (for 1000U)

Cat. No.: TSDSAP-5k (for 5000U)

Description

Thermostable dsAP Nuclease is a heat-stable endonuclease derived from thermophilic bacteria. It specifically recognizes abasic (AP) sites in double-stranded DNA and cleaves the first phosphodiester bond at the 5' end of the AP site, generating a 3' hydroxyl and a 5' deoxyribose phosphate (dRP) terminus.

This enzyme is active exclusively on AP-containing double-stranded DNA (dsDNA) and exhibits no activity on single-stranded DNA (ssDNA), dsDNA without AP sites, or AP-containing ssDNA.

Its optimal activity temperature is 60–70°C, with progressive inactivation occurring above 85°C.

Unit Definition 

One unit is defined as the amount of enzyme required to cleave 1 pmol of AP-containing double-stranded oligonucleotide in a 10 µl reaction system at 60°C within 15 minutes.

Storage 

Store at -20°C for up to two years.