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Cat. No.: TNMB-2 (for 2ml)

Cat. No.: TNMB-10 (for 10ml)

Cat. No.: TNMB-50 (for 50ml)

Description

TED-Ni His-tag protein purification magnetic beads, also known as TED-Ni agarose beads (Magarose), TED-Ni beads, or TED nickel beads, are developed by covalently coupling tris(carboxymethyl)ethylenediamine (TED) to agarose magnetic beads. Subsequently, divalent nickel ions (Ni2+) are chelated at five binding sites of TED to create beads that can selectively bind proteins containing His-tags. This specificity allows them to be used for the purification, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) of proteins or protein complexes with His-tags from lysates of animals, plants, or microorganisms, as well as samples like cell lysates, serum, and ascites. The product can withstand 6M HCl or 8M urea, 20mM chelating agent EDTA, or reducing agent DTT.

Commonly used tags include His-tag, Flag-tag, Myc-tag, HA-tag, and GST-tag. Fusion expression with these tags facilitates the convenient detection of the target protein and proteins interacting with it, as well as the purification of the target protein.

The His-tag consists of a peptide composed of six consecutive histidine residues (HHHHHH). Through genetic recombination, the nucleotide sequence of the His-tag is connected to the 5' or 3' end of the target gene, resulting in the expression of the target protein with a fused His-tag. His-tag advantages include its small molecular weight (0.84 kDa), minimal interference with the target protein's function, and compatibility with various protein expression systems. The His-tag has been widely applied in protein expression, purification, identification, interaction, and functional studies. While nickel columns are generally used for His-tag protein purification, for applications involving small-scale purification or immunoprecipitation of proteins or protein complexes with His-tags, His-tag protein purification agarose beads are simpler and more convenient.

This product is developed based on immobilized metal affinity chromatography (IMAC) technology. A nickel ion has six coordination sites, and the bridge connecting it to agarose or agarose magnetic beads is typically iminodiacetic acid (IDA), nitrilotriacetic acid (NTA), or tris(carboxymethyl)ethylenediamine (TED). IDA, NTA, and TED have 3, 4, and 5 coordination sites with nickel ions, respectively. The number of sites available for histidine tag binding is 3, 2, and 1, respectively. TED exhibits weaker binding capacity with His-tagged proteins, but it has strong specificity and good tolerance in high concentrations of chelating agents, reducing agents, and alkaline environments. In contrast, IDA can bind more His-tagged proteins but with weaker specificity. NTA's interaction with His-tagged proteins falls between the two.

TED-Ni Magnetic Agarose Beads for His-Tag Protein Purification specifically bind to His-tagged fusion proteins and can be easily applied to the purification or immunoprecipitation of proteins or protein complexes with His-tags using magnetic separation devices like magnetic racks.

Features

• This product exhibits high stability, strong specificity, and a high binding capacity for the target protein. Compared to most similar products domestically and internationally, this product has a high nickel ion coordination density, and nickel ions almost do not dissociate, resulting in strong specificity in binding to proteins with His-tags. Each milliliter of the suspension contains approximately 25% agarose magnetic beads precipitation, with no less than >30 μmol/ml of TED-Ni2+. Typically, each milliliter of beads (100% beads) can bind to >10mg of His-tagged fusion protein, with specific maximum binding capacities related to the size of the tagged protein and its molecular weight.
• This product can bind to various forms of His-tagged proteins, specifically binding to N-terminal His fusion proteins (His-Protein) and C-terminal His fusion proteins (Protein-His).
• The binding of the target protein is fast with this product. It utilizes nickel ions chelated with TED, with a particle size around 100 μm. The adsorption process for His proteins is typically completed within 30 minutes, and the purification or immunoprecipitation of the target protein can be accomplished within 60 minutes. Shortening the operation time effectively avoids degradation or denaturation of the target protein during prolonged operations, ensuring the activity of the target protein.
• This product allows efficient elution of His-tagged proteins. Based on the structure, biological function, and subsequent application requirements of the target protein, this product utilizes imidazole for multiple elution steps. The elution process is short, with high efficiency.

Storage

Store at -20ºC, valid for two years. Store at 4ºC, valid for one year.

Related: 

• TED-Ni Magnetic Agarose Beads for His-Tag Protein Purification
• IDA-Ni Magnetic Agarose Beads for His-Tag Protein Purification
• NTA-Ni Magnetic Agarose Beads for His-Tag Protein Purification

Only for research and not intended for treatment of humans or animals

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