NTA-Ni Magnetic Agarose Beads for His-Tag Protein Purification
$99.00 - $939.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: NNMB-2 (for 2ml)
Cat. No.: NNMB-10 (for 10ml)
NTA-Ni Magnetic Agarose Beads for His-Tag Protein Purification, also known as NTA-Ni agarose beads (Magarose), NTA-Ni beads, or NTA nickel beads, are prepared by covalently coupling high-quality nitrilotriacetic acid (NTA) to agarose magnetic beads. The NTA is then used to chelate divalent nickel ions (Ni2+) at four binding sites, resulting in beads that can selectively bind proteins containing His-tags in lysates from animals, plants, or microorganisms, as well as in samples such as cell lysates, serum, and ascites. These beads are employed for the purification, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) of proteins or protein complexes with His-tags. The product can tolerate 6M HCl or 8M urea.
Commonly used tags include His-tag, Flag-tag, Myc-tag, HA-tag, and GST-tag. Fusion expression with these tags facilitates the convenient detection of the target protein and proteins interacting with it. These tags are also useful for the purification of the target protein.
The His-tag consists of a peptide composed of six consecutive histidine residues (HHHHHH). By recombinant DNA technology, the nucleotide sequence of the His-tag is fused to the 5' or 3' end of the target gene, resulting in the expression of the target protein with a fused His-tag. The advantages of the His-tag include its small molecular weight (0.84 kDa), minimal interference with the target protein's function, and compatibility with various protein expression systems. His-tagged proteins can be easily detected using His-tag antibodies, and purification can be achieved using His-tag protein purification agarose beads or conventional His-tag protein purification media.
The product described is developed based on immobilized metal affinity chromatography (IMAC) technology. Nickel ions, with six coordination sites, are connected to agarose or agarose magnetic beads through chelating agents such as iminodiacetic acid (IDA), nitrilotriacetic acid (NTA), or Tris (carboxymethyl) ethylenediamine (TED). NTA-Ni beads, having 4 coordination sites, exhibit intermediate binding strength between IDA and TED, making them suitable for efficient purification and convenient applications.
NTA-Ni Magnetic Agarose Beads for His-Tag Protein Purification specifically bind to His-tagged fusion proteins and can be easily applied to the purification or immunoprecipitation of proteins or protein complexes with His-tags using magnetic separation devices like magnetic racks.
- This product exhibits high stability, strong specificity, and a high binding capacity for the target protein. In comparison to most similar products, this product has a higher nickel ion coordination density, resulting in lower nickel ion dissociation. It demonstrates strong specificity in binding to proteins with His-tags. Each milliliter of the suspension contains approximately 25% agarose magnetic beads precipitation, with no less than 30 μmol/ml of NTA-Ni2+. Typically, each milliliter of beads (100% beads) can bind to >20mg of His-tagged fusion protein, with specific maximum binding capacities related to the size of the tagged protein and its molecular weight.
- This product can bind to various forms of His-tagged proteins, specifically binding to N-terminal His fusion proteins (His-Protein) and C-terminal His fusion proteins (Protein-His).
- The binding of the target protein is rapid with this product. It utilizes nickel ions chelated with NTA, with a particle size around 100 μm. The adsorption process for His proteins is typically completed within 30 minutes, and the purification or immunoprecipitation of the target protein can be accomplished within 60 minutes. Shortening the operation time effectively avoids degradation or denaturation of the target protein during prolonged operations, ensuring the activity of the target protein.
- This product allows efficient elution of His-tagged proteins. Based on the structure, biological function, and subsequent application requirements of the target protein, this product utilizes imidazole for multiple elution steps. The elution process is short, with high efficiency.
Store at -20ºC, valid for two years. Store at 4ºC, valid for one year.
- TED-Ni Magnetic Agarose Beads for His-Tag Protein Purification
- IDA-Ni Magnetic Agarose Beads for His-Tag Protein Purification
- NTA-Ni Magnetic Agarose Beads for His-Tag Protein Purification
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory