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Cat. No.: T7ARCA-10 (for 10T)

Cat. No.: T7ARCA-50 (for 50T)

Description

Efficient translation of most eukaryotic mRNAs requires a 7-methylguanosine cap at the 5’ end and a Poly(A) tail at the 3’ end. The T7 ARCA in vitro mRNA Synthesis Kit enables the rapid synthesis of fully capped and tailed mRNA in vitro.

During transcription, T7 RNA polymerase incorporates an anti-reverse cap analog (ARCA) at the mRNA’s 5’ end. After a brief incubation with DNase I, the template DNA is removed, followed by Poly(A) polymerase extension to add a Poly(A) tail to the capped mRNA.

The resulting RNA is suitable for a wide range of downstream applications, including RNA structure and function studies, nucleic acid enzyme biochemistry research, RNase protection assays, blot hybridization, antisense RNA and RNA interference (RNAi) experiments, microarray analysis, microinjection, in vitro translation, and RNA vaccine development.

T7 RNA polymerase recognizes the T7 promoter sequence (TTCTAATACGA CTCACTATAGG) and initiates transcription from the -2 position G base. This kit enables high-efficiency transcription from minimal sample input, yielding over 30 μg of mRNA per reaction, with transcription lengths exceeding 4000 nucleotides. Over 80% of the transcription products contain a 7-methylguanosine cap, and the Poly(A) tail length exceeds 80 nucleotides.

Components

• 5×ARCA/NTP mix
• 5×T7 RNA Pol Buffer
• T7 ARCA Enzyme Mix
• 10×EPAP Reaction Buffer
• Poly(A) Polymerase
• RNase Free DNase I
• RNase Free H2O

Features

• Completely free from bacterial DNA.
• High transcriptional activity, NTP concentration range: 0.2-10 mM.
• The T7 promoter is short and simple, making it easy to design and synthesize.

Storage 

Store at -20°C for up to 2 years. Avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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