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Cat. No.: SGMRT-100 (for 100μg)

Cat. No.: SGMRT-200 (for 200μg)

Description

Sequencing Grade Modified Recombinant Trypsin is produced by expression in Escherichia coli and has an amino acid sequence identical to porcine pancreatic cationic trypsin. It is free from contaminating enzymes such as chymotrypsin, and does not contain or require TPCK to inhibit other enzyme activities. It exhibits high activity and specificity. Trypsin is prone to autolysis, which can affect mass spectrometry analysis. The methylation modification and mutation ensure that our trypsin does not undergo autolysis, thereby eliminating the activity of false chymotrypsin generated by autolysis.

Applications

Sequencing Grade Modified Recombinant Trypsin exhibits enzymatic properties identical to animal-derived trypsin. It can be used as a substitute for trypsin with several advantages, including no enzymatic contamination, high stability, no autolysis, and high activity. It is suitable for specific protein digestion, protein sequencing, peptide mapping, proteomics research, and trypsin digestion of peptides on 2-D gels.

Protein Information

• Physical appearance: White or off-white powder
• Biological activity: ≥ 4500 USP units/mg pro.
• Unit definition: One USP unit is defined as the amount of trypsin that increases the absorbance at 253nm by 0.003 per minute at 25°C, pH 7.6, in a reaction system of 3.2ml (1cm light path) using BAEE as the substrate.
• Purity: ≥ 95% by HPLC
• Protein content: Not specified
• Formulation: Lyophilized from an additive-free solution.
• Recommended usage: Dissolve or dilute in 50mM Acetic Acid (HAc). For use, dilute in a trypsin digestion buffer of 50mM NH4HCO3 or 50mM Tris-HCl (pH 7.0-8.0) with 1mM CaCl2. The recommended ratio of recombinant trypsin to target protein is 1:20-1:100, and the optimal pH is 7.0-8.0.
• Stability: The lyophilized form of Sequencing Grade Modified Recombinant Trypsin is stable for 24 months when stored at 2-8°C. After reconstitution with 50mM HAc or 1mM HCl, it can be stored at -20°C or -80°C without degradation or loss of activity, even after five freeze-thaw cycles.
• Amino acid sequence: IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN. Note: This amino acid sequence is provided for reference and may have some variations in actual amino acid length or sequence.

Advantages

• Non-animal sourced: Recombinant production without viral contamination from exogenous sources, and no animal-derived raw materials used in the manufacturing process.
• Quality stability: Bulk production ensures stable and consistent batch-to-batch manufacturing. There are no differences in quality between product batches.
• High purity: High specific activity, with residual levels of host proteins below the requirements for biopharmaceuticals.
• Lyophilized powder: Easy to store and transport.

Precautions

• This product is intended for scientific research by professionals only and is not to be used for clinical diagnosis or treatment. It is not suitable for use in food or drugs and should not be stored in residential areas.
• For your safety and health, please wear appropriate laboratory attire and disposable gloves while handling.

Storage

Store at 4°C or below, valid for two years. Due to protein inactivation during each freeze-thaw cycle, after the initial reconstitution into the appropriate concentration, it should be aliquoted and stored at -20°C or below to avoid repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory