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Cat. No.: RNGG-2500 (for 2500U)

Cat. No.: RNGG-12500 (for 12500U)

Recognition Site

GG
5'...G ↓ G ...3'

Description

RNase GG is derived from a thermophilic archaeon and obtained through recombinant expression and purification. RNase GG is an ribonuclease endonuclease that specifically recognizes and cleaves GG sites in single-stranded (ssRNA) or double-stranded RNA (dsRNA), but does not cleave ssDNA or dsDNA. RNase GG can also efficiently cleave GG sites within secondary structures, making it highly suitable for analyzing RNAs with complex structures. RNase GG is extremely thermostable, maintaining activity between 37–95°C, with optimal activity at 60–70°C. The enzymatic activity of RNase GG is independent of metal ions and is compatible with most buffers commonly used in RNA research.​

Components

• RNase GG
• 10× RNase GG Buffer

Activity Definition

One unit (U) of activity is defined as the amount of enzyme required to completely cleave 2 pmol of a 40-nt RNA substrate containing a single G/G site at 37°C within 30 minutes.

Recommended Reaction Conditions​

• ​Buffer:​​ 1× RNase GG Buffer
• ​Incubation Temperature:​​ 60°C

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Inactivation Conditions​

• ​Final Concentration:​​ 1% SDS
• ​Temperature & Time:​​ 95°C for 3 minutes

Quality Control

• Protein Purity​: The protein purity is determined to be no less than 95% by SDS-PAGE gel electrophoresis.
• ​Non-specific DNA Endonuclease Activity​: Under 37°C, 50 U of RNase GG is incubated with 200 ng of supercoiled plasmid DNA in a 20 μl reaction system for 4 hours. Agarose gel electrophoresis analysis shows that less than 20% of the plasmid DNA is converted into nicked or linear forms.
• ​DNase Activity​: Under 37°C, 50 U of RNase GG is incubated with 15 ng of double-stranded DNA fragments in a 20 μl reaction system for 16 hours. Agarose gel electrophoresis analysis shows no change in the double-stranded DNA fragments.
• ​RNase Activity​: Under 37°C, 50 U of RNase GG is incubated with RNA lacking GG sites in a 10 μl reaction system for 1 hour. Polyacrylamide gel electrophoresis analysis shows no change in the RNA.

Storage

-20°C.

RNA Endonuclease:

• RNase GG
• MazF enzyme