All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: RVC-2 (for 2ml)

Cat. No.: RVC-10 (for 10ml)

Description

Ribonucleoside Vanadyl Complexes (RVC) are commonly used as inhibitors of ribonucleases (RNases), also known as RNAase inhibitors, with a concentration of 200 mM. They are widely used in the separation, purification, and detection processes of various RNA samples to prevent RNA degradation.

This product is prepared by an optimized method that combines equimolar mixtures of four rNTPs with vanadyl oxide, forming stable complexes that non-covalently bind to RNases, thereby inhibiting their activity.

This product is a commonly used RNase inhibitor and can be widely applied in RNA separation, purification, and detection processes where prevention of RNA degradation is required. It can be used as an RNase inhibitor during mRNA purification, as well as in cell lysis and sucrose gradient centrifugation for cell fractionation to inhibit RNase activity. It can also protect RNA probes in in situ hybridization and RNA during DNase I digestion.

This product can inhibit the majority of ribonucleases, except for DNase I, S1 nuclease, and Bacillus cereus ribonuclease. As it does not inhibit DNase I, it can be used as an RNase inhibitor when removing DNA from RNA samples using DNase I digestion.

This product is not suitable for use in in vitro translation systems.

It has been reported that RVC at concentrations above 0.4 mM has some inhibitory effects on PCR polymerases. Therefore, this reagent is not recommended for direct use in reverse transcription reactions. Instead, RNase inhibitor (RNasin) can be used. Alternatively, RVC can be removed from the sample using the method mentioned later and then used in PCR reactions.

In many cases, it is not necessary to remove RVC from RNA samples, and they can be directly used for subsequent applications such as gel electrophoresis and functional assays. For example, mRNA purified using this product without removing RVC can be directly used for injection experiments in frog eggs.

If it is necessary to remove RVC from RNA samples, direct extraction using an equal volume of phenol can be performed. If the phenol contains the antioxidant 8-hydroxyquinoline, the color of phenol will turn black after extracting the RVC-containing RNA sample. After extracting the sample with phenol 2-4 times, the color of phenol will no longer significantly change, indicating that the RVC has been adequately removed. Alternatively, before RNA precipitation, adding 10 times the molar amount of EDTA as the RVC can fully decompose and inactivate the RVC, allowing removal during RNA precipitation of the already decomposed and inactivated RVC.

The inhibitory effect of RVC on RNases becomes stronger with higher concentrations. A concentration of 20 mM RVC can completely inhibit 0.00025 U/ml of RNase A. This product has a concentration of 200 mM and is typically recommended for a final concentration of 20 mM in cell lysis buffers. However, the appropriate concentration can be selected based on experience or literature references.

Storage

The minimum shelf life is 1 year at -20°C.

Note

• It is recommended to aliquot upon the first use to avoid repeated freeze-thaw cycles.
• The activity of RVC can be inhibited by the detergent SDS and the chelating agent EDTA, so they should not be used simultaneously with RVC.
• This product is intended for scientific research purposes by professionals only and should not be used for clinical diagnosis or treatment. It should not be used for food or drugs, and should not be stored in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory