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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

Synonyms    

CDF; D factor; DIA; DI; differentiation inhibitory activity; differentiation stimulating factor; emfilermin; differentiation-stimulating factor; HILDA; cholinergic differentiation factor; leukemia inhibitory factor; melanoma-derived LPL inhibitor; MLPLI; hepatocyte-stimulating factor Ⅲ; rMuLIF; recombinant murine leukemia inhibitory factor


Source    

Recombinant protein containing the truncated mouse leukemia inhibitory factor is expressed in E. coli.


Purity    

Sterile, ≥ 95% by SDS-PAGE.


Biological Activity    

Fully biologically active when compared to standard. The specific activity is determined by adding LIF to maintain the morphology and inhibit the differentiation of mouse embryonic stem cell (E14 cell) . And the larger in embryoid body (EB)’s diameter (um) , the stronger biological activity.


Physical Appearance    

Sterile filtered white lyophilized (freeze-dried) powder


Formulation    

Lyophilized from a 0.2μM filtered concentrated solution in 1X PBS, pH7.4.


Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.


Category    

Cytokine


Background    

Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass [1]. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78% a.a. sequence identity with Human LIF

Only for research and not intended for treatment of humans or animals

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