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Cat. No.: RPNGF-50 (for 50μl)

Cat. No.: RPNGF-250 (for 250μl)

Description

Rapid PNGase F is an amidase that cleaves N-linked glycans from glycoproteins. It acts on high-mannose, hybrid and complex-type oligosaccharides, cleaving the bond between the innermost N-acetylglucosamine (GlcNAc) and asparagine residue. A glycerol-free version of Rapid PNGase F is also available to achieve optimal performance in HPLC workflows.

Our Rapid PNGase F is recombinantly expressed and purified via multi-step chromatographic procedures. A volume of 1–2 μL enzyme is recommended per reaction.

Unit Definition

One unit is defined as the amount of enzyme required to remove greater than 95% of carbohydrates from 10 µg denatured RNase B within 60 minutes at 37 °C in 1× PNGase reaction buffer. Glycosylated RNase B has a molecular weight of approximately 17,000 Da; deglycosylated RNase B is ~13,683 Da.

Features & Applications

• Enzymatic deglycosylation to remove N-linked glycans from glycoproteins
• Rapid PNGase F can hydrolyze nearly all common N-linked glycans on glycopeptides and glycoproteins

Storage

Stable for 2 years at −20 °C. Avoid repeated freeze-thaw cycles.

Related: 

• PNGase F
• PNGase F (with His-tag)
• PNGase F (with His-tag, Glycerol Free)
• Rapid PNGase F
• PNGase F Deglycosylation Kit with Magnetic Beads
• Rapid PNGase F Deglycosylation Kit (Denaturing Method)
• Rapid PNGase F Deglycosylation Kit (Native Method)

Only for research and not intended for treatment of humans or animals

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