Protease Inhibitor Cocktail (for Purification of His-tagged Proteins, 100X)
$115.00 - $450.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: PICPH-1 (for 1ml)
Cat. No.: PICPH-5 (for 5ml)
Description
Protease Inhibitor Cocktail (for Purification of His-tagged Proteins, 100X) is a mixture of protease inhibitors used for His-tag protein purification (100mM AEBSF, 5mM Bestatin, 1.5mM E64, 2mM Pepstatin A, and 0.2mM Phosphoramidon in DMSO).
Cell or tissue extracts contain many endogenous proteases and phosphatases, which can easily lead to protein degradation or dephosphorylation in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease and phosphatase inhibitors to the extracts is an effective method to prevent protein degradation and dephosphorylation.
This product is an optimized and tested protease inhibitor cocktail for His-tag protein purification, containing broad-spectrum serine, cysteine, and acidic protease inhibitors, as well as aminopeptidase inhibitors.
This product is convenient to use. Typically, a 1:100 ratio of the Protease Inhibitor Cocktail (for Purification of His-tagged Proteins, 100X) is added to the lysis buffer for His-tag protein purification, effectively inhibiting protein degradation.
Different types of protein extracts contain different types of proteases. For example, mammalian cell extracts usually contain high levels of serine proteases, bacterial cell extracts often contain more serine and metalloproteases, mammalian tissue extracts mainly contain serine, cysteine, and metalloproteases, and plant extracts commonly contain large amounts of serine and cysteine proteases, with some also containing aspartic proteases. Therefore, we provide different types of protease and phosphatase inhibitor cocktails for different types of sample protein extraction.
This product can effectively inhibit various protease activities during His-tag protein purification, such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartic proteases, and metalloproteases. Among them, AEBSF is a water-soluble, irreversible serine protease inhibitor with an inhibition constant similar to PMSF and DFP, effectively inhibiting proteases such as trypsin, chymotrypsin, plasmin, kallikrein, and thrombin. As a substitute for PMSF and DFP, AEBSF has lower toxicity, better water solubility, and stability in aqueous solutions. Bestatin is a reversible aminopeptidase inhibitor; E64 is an irreversible cysteine protease inhibitor; Pepstatin A is a reversible aspartic protease inhibitor; and Phosphoramidon is a reversible metalloprotease inhibitor.
Storage
Store at -20°C for one year. Store at 4°C for one month. Store at room temperature for one week.
Precautions
- This product can be aliquoted and stored appropriately. It should be added to lysis buffer or other suitable solutions just before use.
- The protease inhibitors contained in this product are harmful to humans. Handle with care and use effective protection to avoid direct contact with the body or inhalation.
- This product is for scientific research use by professionals only. It is not for clinical diagnosis or treatment, not for food or drug use, and should not be stored in ordinary residences.
- For your safety and health, wear a lab coat and disposable gloves when handling this product.
Related:
- Phosphatase Inhibitor Cocktail A (50X)
- Phosphatase Inhibitor Cocktail B (50X)
- Phosphatase Inhibitor Cocktail C (50X)
- Phosphatase Inhibitor Cocktail D (50X)
- Deacetylase Inhibitor Cocktail (100X)
- Protease Inhibitor Cocktail (100X)
- Protease Inhibitor Cocktail (MS-SAFE, 50X)
- Protease Inhibitor Cocktail (for Mammalian Cell and Tissue Extracts, 100X)
- Protease Inhibitor Cocktail (for Plant Cell and Tissue Extracts, 100X)
- Protease Inhibitor Cocktail (for Fungal and Yeast Extracts, 100X)
- Protease Inhibitor Cocktail (for Bacterial Cell Extracts, 100X)
- Protease Inhibitor Cocktail (for Purification of His-tagged Proteins, 100X)
- Protease and Phosphatase Inhibitor Cocktail (50X)
- Protease and Phosphatase Inhibitor Cocktail (MS-SAFE, 50X)
- Protease and Phosphatase Inhibitor Cocktail (for Mammalian Cell and Tissue Extracts, 50X)
- Protease and Phosphatase Inhibitor Cocktail (for Plant Cell and Tissue Extracts, 50X)
- Protease and Phosphatase Inhibitor Cocktail (for Fungal and Yeast Extracts, 50X)
- Protease and Phosphatase Inhibitor Cocktail (for Bacterial Cell Extracts, 50X)
Only for research and not intended for treatment of humans or animals
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