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Cat. No.: PICPT-1 (for 1ml)

Cat. No.: PICPT-5 (for 5ml)

Description

Protease Inhibitor Cocktail (for Plant Cell and Tissue Extracts, 100X) is a mixture of protease inhibitors used for protein extraction from plant cells or tissues (200mM AEBSF, 10mM Bestatin, 3mM E64, 2mM Leupeptin, 2mM Pepstatin A, and 500mM Phenanthroline in DMSO).

Plant cell or tissue extracts contain many endogenous proteases and phosphatases, which can easily lead to protein degradation or dephosphorylation in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease and phosphatase inhibitors to the extracts is an effective method to prevent protein degradation and dephosphorylation.

This product is an optimized and tested protease inhibitor cocktail for protein extraction from plant cells or tissues, containing broad-spectrum serine, cysteine, and acidic protease inhibitors, as well as aminopeptidase inhibitors.

This product is convenient to use. Typically, a 1:100 ratio of the Protease Inhibitor Cocktail (for Plant Cell and Tissue Extracts, 100X) is added to the lysis buffer for protein extraction from plant cells or tissues, effectively inhibiting protein degradation.

Different types of protein extracts contain different types of proteases. For example, mammalian cell extracts usually contain high levels of serine proteases, bacterial cell extracts often contain more serine and metalloproteases, mammalian tissue extracts mainly contain serine, cysteine, and metalloproteases, and plant extracts commonly contain large amounts of serine and cysteine proteases, with some also containing aspartic proteases. Therefore, we provide different types of protease and phosphatase inhibitor cocktails for different types of sample protein extraction.

This product can effectively inhibit various protease activities in plant cell or tissue extracts, such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartic proteases, and metalloproteases. Among them, AEBSF is a water-soluble, irreversible serine protease inhibitor with an inhibition constant similar to PMSF and DFP, effectively inhibiting proteases such as trypsin, chymotrypsin, plasmin, kallikrein, and thrombin. As a substitute for PMSF and DFP, AEBSF has lower toxicity, better water solubility, and stability in aqueous solutions. Bestatin is a reversible aminopeptidase inhibitor; E64 is an irreversible cysteine protease inhibitor; Leupeptin is a reversible serine and cysteine protease inhibitor; Pepstatin A is a reversible aspartic protease inhibitor; and Phenanthroline is a reversible metalloprotease inhibitor.

Storage

Store at -20°C for one year. Store at 4°C for two months. Store at room temperature for two weeks.

Precautions

• This product can be aliquoted and stored appropriately. It should be added to lysis buffer or other suitable solutions just before use.
• Due to the presence of the metalloprotease inhibitor Phenanthroline, which can form red or purple chelates with divalent metal ions like ferrous ions, this product may appear light pink. If the lysis buffer or sample contains a high concentration of divalent metal ions like ferrous ions, a deeper red color may appear, but this does not affect its use.
• The protease inhibitors contained in this product are harmful to humans. Handle with care and use effective protection to avoid direct contact with the body or inhalation.
• This product is for scientific research use by professionals only. It is not for clinical diagnosis or treatment, not for food or drug use, and should not be stored in ordinary residences.
• For your safety and health, wear a lab coat and disposable gloves when handling this product.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory