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Cat. No.: MDAWGA-50 (for 50T)

Cat. No.: MDAWGA-500 (for 500T)

Description

The basic principle of Multiple Displacement Amplification (MDA) relies on random hexamer primers and phi29 DNA Polymerase to carry out amplification under isothermal conditions. During the reaction, the random hexamers anneal arbitrarily to the DNA template, after which phi29 DNA Polymerase initiates synthesis simultaneously at multiple loci across the genome. As replication progresses, the newly synthesized DNA strands displace the complementary strands of the template, which in turn serve as fresh templates for further rounds of amplification. Capable of yielding high-mass, high-quality genomic DNA from trace biological samples, MDA has emerged as the most widely adopted method for single-cell whole genome amplification (WGA).

Components

• Phi29 DNA polymerase
• 10x Reaction Buffer
• Random Primers
• 10 mM dNTPS
• Enhancer Buffer
• Diluent buffer

Storage

Store at -20°C. Upon receipt, aliquoting is highly recommended to avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data