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Cat. No.: MPERII-2 (for 2ml)

Cat. No.: MPERII-10 (for 10ml)

Description

Mycoplasma Prophylactic and Elimination Reagent II is a reagent used to prevent and eliminate mycoplasma contamination commonly found in cultured cells. This reagent can prevent mycoplasma contamination and can also be used to remove existing mycoplasma contamination in cells. The main components of this mycoplasma prevention and elimination reagent are tetracycline antibiotics and diterpenoid antibiotics.

Mycoplasma is the smallest and simplest prokaryote. Mycoplasma has the following characteristics:

• Mycoplasma lacks a cell wall structure, so many common antibiotics targeting the cell wall, such as penicillin or β-lactam antibiotics, are ineffective against mycoplasma.
• Mycoplasma sizes range between bacteria and viruses, approximately 0.2-0.8μm, so some mycoplasma can pass through 0.22μm filters, making conventional filtration ineffective.
• Many mycoplasmas rely on the host for nutrients due to their limited biosynthetic capabilities, often adhering to or dispersing between cell surfaces.

These characteristics of mycoplasma pose a risk of contamination during cell culture, and mycoplasma contamination has become a global issue. Mycoplasma contamination can severely affect cell status, altering gene expression and metabolic characteristics, leading to slowed cell growth, abnormal differentiation, and death, significantly impacting cell function. These factors can seriously affect the reliability and reproducibility of experimental results, making the prevention and elimination of mycoplasma contamination crucial.

This mycoplasma prevention and elimination reagent contains two effective antibiotics against mycoplasma: tetracycline antibiotics and diterpenoid antibiotics. These two mycoplasma antibiotics inhibit protein synthesis through different mechanisms:

• Tetracycline antibiotics bind to the A site of the 30S ribosomal subunit, preventing the binding of aminoacyl-tRNA at this site, inhibiting peptide chain elongation, and thus blocking protein synthesis.
• Diterpenoid antibiotics bind to the 50S ribosomal subunit, inhibiting peptidyl transferase activity, ultimately inhibiting protein synthesis.

This reagent is effective only against mycoplasma and bacteria, and does not affect eukaryotic cells, having minimal impact on the normal growth and function of eukaryotic cells.

For a comparison and selection of our four types of mycoplasma prophylactic and elimination reagents, please refer to the table below:

Mycoplasma Removal Reagent and Mycoplasma Removal Reagent Plus are both single antibiotics in powder form. They are slightly inconvenient to use, but you can measure out small amounts according to your needs. If stored at -20°C, the powder products can last longer. Additionally, if resistance develops to a single antibiotic, it is relatively simple to switch to another antibiotic, which usually yields better results.

Mycoplasma Prophylactic and Elimination Reagent I and Mycoplasma Prophylactic and Elimination Reagent II are pre-prepared solutions, making them more convenient to use, but they must be stored at -20°C. Since these two reagents are combination antibiotics, they generally have a stronger effect in inhibiting or removing mycoplasma. However, if resistance develops, subsequent handling can be more troublesome, and it is more likely that mycoplasma resistance will persist even after switching to another prevention and removal reagent.

This product is easy to use. Simply add it directly to the culture medium at a dilution ratio of 1:500 (for prevention) or 1:100 (for removal). Each milliliter of this product can prepare 500ml of prevention culture medium or 100ml of removal culture medium.

Storage

Store at -20°C, valid for two years.

Precautions

• Avoid repeated freezing and thawing of this reagent. If used multiple times, please consider appropriate aliquoting.
• If crystals or precipitates are observed after thawing, it does not affect the usage. Shaking can help dissolve the crystals or precipitates.
• It is recommended to regularly test for mycoplasma using mycoplasma detection kits, such as our Luminescence Mycoplasma Detection Kit, Mycoplasma PCR Detection Kit, or Mycoplasma Staining Detection Kit.
• This reagent can effectively inhibit or remove various mycoplasmas, but it cannot guarantee the inhibition or removal of all types of mycoplasmas encountered in actual experimental operations. Although the likelihood of mycoplasma developing resistance to this reagent is low, long-term use may still result in resistance. If the reagent is not effective, consider using our other mycoplasma removal reagents.
• This product is toxic to specific organs such as the respiratory and reproductive systems and may be teratogenic or carcinogenic. Handle with care and use effective protection to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or drugs, and should not be stored in ordinary residences.
• For your safety and health, please wear a lab coat and disposable gloves when handling.