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Cat. No.: MRDE-2k (for 2KU)

Cat. No.: MRDE-10k (for 10KU)

Description

mRNA Decapping Enzyme is a decapping enzyme that catalyzes the cleavage and removal of the 7‑Methylguanosine Cap (m7G) structure at the 5' end of mRNA, generating a 5' monophosphate end and 7‑Methylguanosine 5'‑Diphosphate (m7GDP). This enzyme can decap mRNAs of various lengths and exhibits equivalent removal efficiency toward Cap‑0 and Cap‑1 cap structures. It can also convert the 5' triphosphate end of mRNA to a 5' monophosphate end, albeit with relatively low conversion efficiency. RNA with a 5' monophosphate modification is applicable to a variety of downstream experiments, including 5' RNA Ligase‑mediated Rapid Amplification of cDNA Ends (5' RLM‑RACE), RNA‑seq, and 5'→3' exonuclease digestion.

Applications

Can be used as an alternative to Tobacco acid pyrophosphatase (TAP) to remove Cap‑0 and Cap‑1 cap structures from mRNAs of various lengths. The resulting 5'‑monophosphorylated RNA can be used in 5' RLM‑RACE and RNA‑seq.

Source

Recombinant protein expressed in Escherichia coli, with the expressed gene being the mRNA Decapping Enzyme gene.

Definition of Activity Unit

One unit is defined as the amount of mRNA Decapping enzyme required to convert 50% of a 500 nM m7G‑capped substrate to a 5'‑monophosphorylated form in a total reaction volume of 20 µl within 1 hour at 37 °C.
 

Purity

Free of endonucleases, exonucleases, and RNases.

Storage Conditions

Store at -20 °C. Stable for at least 2 years. No significant loss of enzymatic activity after storage at room temperature or 37 °C for 3 days.

Precautions

• Although the product shows no significant decrease in activity after storage at room temperature or 37 °C for 3 days, it should be kept on ice during use and immediately returned to -20 °C storage after use.
• Since the experiment involves RNA manipulation, strict RNA-handling protocols must be followed to avoid RNase contamination.
• Laboratory consumables such as pipette tips and microcentrifuge tubes used in the experiment must be RNase‑free or treated with DEPC.
• Appropriate protocols may be chosen according to specific applications. Additional reagents such as RNase Inhibitor and ultrapure water may be required.
• This product is for research use only by qualified personnel.
• It is not intended for clinical diagnosis or therapy, nor for use in food or pharmaceutical products. Do not store in ordinary residential premises.
• For your safety and health, please wear a lab coat and disposable gloves during operation.