mRNA Cap 2'- O-Methyltransferase
$44.00 - $176.00
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Cat. No.: C2OM-2k (for 2KU)
Cat. No.: C2OM-10k (for 10KU)
Description
mRNA Cap 2'-O-Methyltransferase (mRNA 2'-O-MTase) is an mRNA capping enzyme that converts the Cap-0 structure into a Cap-1 structure. Utilizing S-adenosylmethionine (SAM) as a methyl donor, it transfers a methyl group to the 2'-O position of the first nucleotide adjacent to the Cap-0 structure (m7GpppNp) at the 5' end of the mRNA, resulting in the formation of Cap-1-modified mRNA (m7GpppNmp). This modification enhances mRNA stability and translation efficiency, improving expression levels after microinjection and transfection. Additionally, Cap-1 helps mRNA evade certain innate immune responses in cells.
mRNA Cap 2'-O-Methyltransferase specifically targets N7-methylguanosine-capped mRNA (m7GpppNp), meaning it requires an existing Cap-0 structure for activity. It does not modify mRNA with 5' terminal structures such as pN, ppN, pppN, or GpppN. Typically, Faustovirus Capping Enzyme is used to generate Cap-0 mRNA, followed by mRNA Cap 2'-O-Methyltransferase to introduce the Cap-1 modification. Alternatively, both enzymes can act synergistically to generate Cap-1 mRNA in a single step.
Applications
- Capping mRNA for in vivo or in vitro translation.
- The 2'-O methylation in the Cap-1 structure helps mRNA evade certain innate immune responses in cells.
- Enhances mRNA translation efficiency.
- Improves mRNA expression levels following microinjection and transfection.
Source
Produced by Escherichia coli (E. coli) expression of the vaccinia virus mRNA Cap 2'-O-Methyltransferase gene, followed by purification.
Activity Unit Definition
One unit of mRNA Cap 2'-O-Methyltransferase is defined as the amount of enzyme required to methylate 10 pmoles of an 80-nt-long capped RNA transcript in 1 hour at 37ºC.
Purity
- Free from DNA endonucleases and exonucleases.
- Free from RNases.
- Free from phosphatases.
Inactivation/Inhibition
Heating at 70ºC for 10 minutes will inactivate mRNA Cap 2'-O-Methyltransferase.
Storage
The minimum shelf life is 2 years at -20°C.
Precautions
- During use, keep the product on an icebox or ice bath, and return it to -20ºC storage immediately after use.
- Since RNA manipulation is involved, strict RNA-handling protocols must be followed to prevent RNase contamination.
- Laboratory consumables such as pipette tips and centrifuge tubes must be RNase-free or treated with DEPC.
- Depending on the specific application, additional reagents such as RNase Inhibitor and ultrapure water may be required.
- S-adenosylmethionine (SAM) is unstable at pH 7-8 and 37ºC. Prepare a fresh working solution immediately before use and keep it on ice to prevent degradation.
- For research use only: This product must not be used for clinical diagnosis, treatment, food, or pharmaceuticals, and must not be stored in residential settings.
- Safety precautions: Wear a lab coat and disposable gloves while handling the product.
Related:
SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.
