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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: C2OM-2k (for 2KU)

Cat. No.: C2OM-10k (for 10KU)

Description

mRNA Cap 2'-O-Methyltransferase is capable of adding a methyl group to the 2'-OH of the first nucleotide adjacent to the 5´ end cap structure of RNA. It utilizes S-adenosylmethionine (SAM) as the methyl donor, leading to the 2’-O-methylation of the cap-O of capped RNA, forming Cap-1. Research has shown that the 2’-O-methylation of the Cap-1 structure can allow mRNA to evade certain immune responses in eukaryotic cells. mRNA Cap 2'-O-Methyltransferase can only methylate RNA with a 5' end cap structure of m7GpppN, and it cannot methylate RNA with 5' ends like pN, ppN, pppN, or GpppN. RNA with an m7GpppN cap structure can be modified by adding a cap analog in vitro transcription reactions or through in vitro capping systems. 

2kU of mRNA Cap 2'-O-Methyltransferase can methylate 400 μg of capped RNA. Our mRNA Cap 2'-O-Methyltransferase is recombinantly expressed and purified through multiple steps.

Activity Definition

One activity unit refers to the amount of enzyme required to methylate 10 pmol of an 80 nt capped RNA transcript in 60 minutes at 37°C in 1× mRNA Cap 2'-O-Methyltransferase reaction buffer.

Activity Assay Conditions

50 mM Tris-HCl (pH 8.0), 5 mM KCl, 1 mM DTT, 1 mM MgCl2, incubation at 37°C.

Applications

• Improves mRNA expression after microinjection and transfection.
• Enhances the translation efficiency of RNA.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.