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Cat. No.: HVN-500 (for 500μl)

Cat. No.: HVN-2k (for 2ml)

Cat. No.: HVN-10k (for 10ml)
 

Synonyms    

VN; V75; VNT; VTN-N; rh-VTN; rhVTN; Vitronectin; S-Protein; Serum-Spreading Factor; Epibolin


Source    

Recombinant protein containing the truncated human vitronectin (62-478aa) is expressed in E. coli.


Concentration    

1mg/ml


Purity    

Sterile, ≥ 95% by SDS-PAGE


Biological Activity    

Used as a substrate for the feeder-free culture of human pluripotent stem cells (PSCs) and has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines such as H9 human embryonic stem cells. Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10μg/ml and 100μl/well), approximately >40% cells will adhere specifically in 30 minutes at 37℃.


Physical Appearance    

Liquid


Formulation    

Liquid in 10mM Sodium Phosphate, 150mM NaCl, 0.1mM DTT, 0.1mM PMSF and 50% Glycerol, pH7.1


Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in ultrapure water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.


Category    

Extracellular Matrix (ECM), Cell Culture, Embryo cell culture


Background    

Vitronectin has well-defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to a variety of macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.

 

Only for research and not intended for treatment of humans or animals

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