HotStart Bst DNA/RNA Polymerase (glycerol-free)
$280.00 - $1,856.00
$2,320.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: HSBGF-1600 (for 1600U)
Cat. No.: HSBGF-16k (for 16KU)
Description
HotStart Bst DNA/RNA Polymerase is a blend of HotStart Bst DNA polymerase and the highly thermostable ThermoStable V reverse transcriptase, which remains active at temperatures up to 65°C. This enzyme mix is well-suited for RNA-based LAMP reactions.
Compared to standard Bst DNA/RNA Polymerase, the HotStart version incorporates a heat-activated aptamer that ensures >90% inhibition of Bst activity at temperatures below 30°C, with complete activation within 1 minute at temperatures above 60°C. This feature allows for reaction setup at room temperature while significantly reducing nonspecific amplification under low-temperature conditions.
Additionally, this enzyme is formulated without glycerol, making it ideal for full-system lyophilized product manufacturing.
Components
- HS Bst DNA/RNA Polymerase (16U/μl)
- 10×IsoAmp Buffer2(Mg2+ free)
- 100mM Mg2+
Storage
For long-term preservation, store below –20°C (stable for up to 2 years). The product tolerates up to 10 freeze–thaw cycles without performance loss, though repeated freeze–thawing should be avoided. Once thawed, it is recommended to store the product at 2–8°C, where it remains stable for up to 1 month.
Additional Notes
- We provide robust and reliable solutions for the study of various diseases (African Swine Fever, COVID-19, etc) based on Bst DNA/RNA Polymerase, please contact us or email tech@sbsbio.com for more details.
- For isothermal amplification of DNA only, please see Bst DNA Polymerase.
- For the lyophilized version, please see PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads.
Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021. Please visit:
https://www.science.org/doi/10.1126/science.acx9701
Instruction: Protocol (Bst DNA/RNA Polymerase, glycerol-free), Protocol (Bst DNA/RNA Lyo Buffer)
Related:
- Bst DNA Polymerase Large Fragment
- Bst DNA Polymerase (comparable to 2.0)
- Bst Polymerase (comparable to 3.0)
- Bst Polymerase (glycerol-free)
- Bst DNA/RNA Polymerase (with superior reverse transcription activity)
- Bst DNA/RNA Polymerase (glycerol-free)
- Bst P DNA/RNA Polymerase (glycerol-free) (with superior reverse transcription activity & hot-start property)
- Bst dU-P DNA/RNA Polymerase (glycerol-free) (fully replaces dTTP with 100% dUTP for effective LAMP contamination control)
Customized Lyophilized Microbead Service
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Viewpoint
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
