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Cat. No.: DSRV-100 (for 100ml)

Cat. No.: DSRV-100 (for 500ml)

Description

BioPreserve™ DNA Stabilization Reagent for Virus (abbreviated as BioPreserve™ Reagent or BioPreserve™), also known as DNA Sample Preservation Solution, DNA Virus Sample Room-Temperature Preservation Solution, or DNA Stabilization Reagent, is a non-toxic and harmless liquid reagent used during virus collection. With simple room-temperature handling, it can rapidly inhibit DNase and stably preserve DNA in virus samples. This product is an inactivating virus stabilization solution, widely used for the stabilization and preservation of DNA in fresh virus samples.

This product has a wide range of applications. It can be used for DNA stabilization and preservation in various virus samples, such as plasma, serum, cell-free body fluids, swabs, and cell culture supernatants.

The effect of this product is basically equivalent to that of liquid nitrogen cryopreservation. It can rapidly penetrate into cells, completely inhibit DNase, prevent DNA degradation, and maintain the integrity of DNA in virus samples, thereby perfectly preserving the gene expression profile of the sample. Like liquid nitrogen, it can also preserve DNA in samples for the long term.

This product is safe and convenient. It is non-toxic and non-irritating, and can be used at room temperature, ensuring safety and convenience during use. Compared with liquid nitrogen, it effectively avoids safety hazards such as frostbite or centrifuge tube rupture caused by ultra-low temperatures, and it is very easy to carry and transport.

This product is suitable for the stabilization and preservation of DNA in ordinary fresh virus samples, and is especially suitable for situations where DNA extraction cannot be performed immediately after sample collection. It is particularly applicable to samples collected in the field or in clinical settings, large-scale sample collection, and samples that cannot be frozen immediately.

The preservation time of samples in this product is related to storage temperature. For example, at 37ºC samples can be stably preserved for at least 7 days, at 4ºC for more than 1 month, and at -20ºC or lower temperatures for long-term preservation. Details are shown in the following table:

Samples stored at -20ºC or -80ºC with this product can undergo 3–5 freeze–thaw cycles without significantly affecting DNA quality. In general, it is recommended that samples preserved with this product be transported or stored short-term at room temperature or 4ºC, and long-term at -20ºC or -80ºC.

Virus samples preserved with this product can be subjected to conventional DNA extraction methods, such as spin column and magnetic bead techniques. During use, simply take 100 µl of liquid sample directly as the material for DNA extraction. A small amount of residual BioPreserve™ reagent will not affect subsequent extraction results.

This product can also be used for the stabilization and preservation of RNA in virus samples. For RNA, the stabilization time is significantly shorter: at 37ºC RNA can be stably preserved for about 1 day, and at room temperature for at least 3–5 days.

Virus samples preserved with this product are inactivated and cannot be used for virus culture or isolation.

A 100 ml package of this product can be used to preserve approximately 100–200 samples, and a 500 ml package can be used to preserve approximately 500–1000 samples.

Storage

Store at room temperature or 4ºC. Valid for two years.

Precautions

• Personnel responsible for specimen collection must follow relevant protective requirements. Subsequent operations should be carried out in a certified Class II biosafety cabinet, in compliance with Biosafety Level 2 or higher standards.
• Virus samples obtained during experiments should be promptly added to BioPreserve™ reagent.
• This product is suitable for the stabilization and preservation of DNA in fresh virus samples. The preservation effect of frozen samples subsequently treated with BioPreserve™ is much poorer than that of fresh samples directly preserved with BioPreserve™. Although this product has some stabilizing effect on frozen samples, it is more suitable for fresh samples.
• When samples stored at -20ºC or -80ºC are thawed, crystallization may occur in the BioPreserve™ reagent. This is a normal phenomenon. The reagent can be used once the crystals are completely dissolved, and this will not affect subsequent DNA extraction.
• This product is intended for use only by professionals in scientific research. It must not be used for clinical diagnosis or treatment, must not be used in food or pharmaceuticals, and must not be stored in ordinary residential housing.
• For your safety and health, please wear a lab coat and disposable gloves when handling.