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Cat. No.: DRSRB-100 (for 100ml)

Cat. No.: DRSRB-100 (for 500ml)

Description

BioPreserve™ DNA/RNA Stabilization Reagent for Blood (abbreviated as BioPreserve™ Reagent or simply BioPreserve™), also known as DNA/RNA Sample Preservation Solution, DNA/RNA Blood Sample Room-Temperature Preservation Solution, or DNA/RNA Stabilization Reagent, is a non-toxic and harmless liquid reagent used during blood collection. With simple room-temperature handling, it can rapidly inhibit DNase/RNase and stably preserve DNA/RNA in blood samples. This product is widely used for the stabilization and preservation of DNA/RNA in fresh blood samples.

This product has a wide range of applications. It can be used for DNA/RNA stabilization and preservation in various blood samples, such as those from mice, rats, rabbits, monkeys, pigs, and other vertebrates.

The effect of this product is basically equivalent to that of liquid nitrogen cryopreservation. It can rapidly penetrate into blood cells, completely inhibit DNase/RNase, prevent DNA/RNA degradation, and maintain the integrity of DNA/RNA in blood samples, thereby perfectly preserving genomic DNA and gene expression profiles. Like liquid nitrogen, it can also preserve DNA/RNA in samples for the long term.

This product is safe and convenient. It is non-toxic and non-irritating, and can be used at room temperature, ensuring safety and convenience during use. Compared with liquid nitrogen, it effectively avoids safety hazards such as frostbite or centrifuge tube rupture caused by ultra-low temperatures, and it is very easy to carry and transport.

This product is suitable for the stabilization and preservation of DNA/RNA in ordinary fresh blood samples, and is especially suitable for situations where DNA/RNA extraction cannot be performed immediately after sample collection. It is particularly applicable to samples collected in the field or in clinical settings, large-scale blood sample collection, and samples that cannot be frozen immediately.

The preservation time of samples in this product is related to storage temperature, and differs between DNA and RNA. For DNA, samples can be stably preserved for at least 7 days at 37ºC, more than 1 month at 4ºC, and long-term at -20ºC or lower temperatures. For RNA, the preservation time is significantly shorter: at 37ºC RNA can be stably preserved for about 1 day, and at room temperature for at least 3–5 days. Details are shown in the following table:

Samples stored at -20ºC or -80ºC with this product can undergo 3–5 freeze–thaw cycles without significantly affecting DNA/RNA quality. In general, it is recommended that samples preserved with this product be transported or stored short-term at room temperature or 4ºC, and long-term at -20ºC or -80ºC.

Blood samples preserved with this product can be subjected to conventional DNA/RNA extraction methods, such as spin column, Trizol, and magnetic bead techniques. During use, add an equal volume of ultrapure water (DNase/RNase-free) or other suitable DNase/RNase-free water, mix thoroughly, centrifuge at 10,000 × g at room temperature for 5 minutes, and carefully remove the supernatant. The pellet or remaining volume can then be treated as an ordinary fresh or frozen sample. A small amount of residual BioPreserve™ reagent will not affect subsequent extraction results.

This product can also be used for the stabilization and preservation of proteins in blood. However, BioPreserve™ causes protein denaturation, so subsequent experiments such as Western blot and SDS-PAGE can be performed, but it is not suitable for assays requiring active enzymes or experiments that depend on maintaining protein activity.

Based on the calculation of using 1 ml of this product for every 200 µl of blood sample, a 100 ml package can be used to preserve approximately 100 blood samples, and a 500 ml package can be used to preserve approximately 500 blood samples.

Storage

Store at room temperature or 4ºC. Valid for two years.

Precautions

• Blood samples obtained during experiments should be appropriately aliquoted and promptly added to BioPreserve™ reagent.
• This product is suitable for the stabilization and preservation of DNA/RNA in fresh blood samples. The preservation effect of frozen samples subsequently treated with BioPreserve™ is much poorer than that of fresh samples directly preserved with BioPreserve™. Although this product has some stabilizing effect on frozen samples, it is more suitable for fresh samples.
• When samples stored at -20ºC or -80ºC are thawed, crystallization may occur in the BioPreserve™ reagent. This is a normal phenomenon. The reagent can be used once the crystals are completely dissolved, and this will not affect subsequent DNA/RNA extraction.
• This product is intended for use only by professionals in scientific research. It must not be used for clinical diagnosis or treatment, must not be used in food or pharmaceuticals, and must not be stored in ordinary residential housing.
• For your safety and health, please wear a lab coat and disposable gloves when handling.