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Cat. No.: BCA-500 (for 500T)

Cat. No.: BCA-2500 (for 2500T)

Description

BCA Protein Quantification Assay Kit is a protein quantification reagent kit based on bicinchoninic acid (BCA), which utilizes colorimetric methods to determine the total protein concentration, characterized by high sensitivity and stability. The principle involves the reduction of Cu^2+ to Cu^+ by proteins in an alkaline medium. The BCA reagent chelates with cuprous ions to form a purple-colored complex with strong absorbance at 562nm. Within a wide range of protein concentrations (20-2000ug/ml), there is a good linear relationship between absorbance and protein concentration. The BCA Protein Quantification Assay Kit is compatible with detergents.

Components

• BCA Reagent A
• BCA Reagent B
• BSA Protein Standard (2mg/ml)

Instruction

1. Draw a standard curve each time the sample concentration is determined to ensure accurate measurement results.
2. Dilute protein standards and test samples with the same diluent (recommended 0.9% NaCl or PBS) to ensure accuracy of results.
3. When feasible, it is recommended to perform duplicate reactions (replicate wells) for each BSA standard and test sample to improve measurement accuracy.
4. Before use, refer to Table 1 to determine that there are no interfering substances in the test sample that exceed the tolerated concentrations, such as EDTA with a maximum tolerated concentration of 10mM.

Storage

BCA reagents A and B should be stored at room temperature. Protein standards should be stored at 4℃ with a shelf life of one year; long-term storage is possible at -20℃.

Related:

• BCA Protein Quantification Assay Kit
• Enhanced BCA Protein Assay Kit
• BCA Protein Assay Kit (High Sensitivity)
• BCA Rapid Protein Assay Kit

Only for research and not intended for treatment of humans or animals

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