Compared with E.coli RNase H, the thermostable RNase H has higher activity above 65℃. Due to its extremely high thermal stability, thermostable Rnase H is an ideal choice for molecular biology applications requiring high temperature reaction conditions. This protein is obtained by recombinant expression and purification of Escherichia coli.
A unit refers to the amount of enzyme required to hydrolyze 1 nmol of nucleic acid substrates from 40 pmol of 25 BP length RNA-DNA hybridization chains in a 50μl reaction system at 37℃ within 20 min.
Stored at -20℃ for 3 years.
10 x Rnase H Buffer
750 mM KCl, 500 mM Tris-HCl (pH 8.3), 30 mM MgCl2, 100 mM DTT.