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Cat. No.: TRNH-200 (for 200U)

Cat. No.: TRNH-2k (for 2,000U)

 Description

Thermostable RNase H is an endoribonuclease that remains active at higher temperatures (above 65°C). It selectively recognizes and cleaves the RNA strand in RNA:DNA hybrids, while the DNA strand remains intact. Thermostable RNase H does not degrade single-stranded or double-stranded RNA or DNA.

Applications

• High-precision RNA structure mapping and mRNA capping rate analysis.
• Removal of mRNA during the synthesis of the second strand of cDNA; improves RT-qPCR efficiency.
• Removal of the Poly(A) tail of mRNA in the presence of Oligo(dT).
• Component in isothermal amplification experiments.

Activity Definition

One unit (U) of enzyme is defined as the amount required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 bp RNA:DNA hybrid in a 50 µl reaction system at 50°C within 20 minutes.

Quality Control

Non-specific Endonuclease Activity Detection

Incubate 5 U of this enzyme with supercoiled plasmid DNA at 37°C for 4 hours. Use DNA electrophoresis to confirm that the plasmid remains unchanged.

DNase Residual Detection

Incubate 5 U of this enzyme with double-stranded DNA substrate at 37°C for 16 hours. Use DNA electrophoresis to confirm that the double-stranded DNA substrate remains unchanged.

RNase Residual Detection

Incubate 5 U of this enzyme with 500 ng of RNA at 37°C for 1 hour. Use agarose gel electrophoresis to confirm that more than 90% of the RNA remains intact.

Host DNA Residual Detection

The residual E. coli host cell DNA in this product is less than 10 copies per 5 U. 

Storage

Stored at -20°C.

Related:

• RNase H
• Thermostable RNase H
• Tth Thermostable RNase H
• Tth Thermostable RNase HII
• Extreme Thermostable RNase HII
• RNase HIII

Only for research and not intended for treatment of humans or animals

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