UNG is mainly used to prevent contamination of PCR amplification products. The principle is: if dUTP is incorporated into DNA instead of dTTP in the PCR reaction, a dU-containing PCR amplification product is formed. The enzyme can selectively break the glycosidic bonds of U groups in single-stranded and double-stranded DNA, and degrade the PCR amplification product.
- Removal of uracil bases from single- or double-stranded DNA
- Removal of residual contamination from PCR
Under standard PCR reaction system, the amount of enzyme degrading 1 g dU-containing single-stranded DNA in 1 hour at 37℃ is one active unit.
Uracil DNA Glycosylase (UNG) is compatible with most PCR polymerase reaction buffers, but its activity is inhibited at high ion concentrations (>100 mM).
20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 50% Glycerol, 0.1% (w/v) Triton X-100, pH 7.5.
Stored at -20 C for 2 years. Avoid repeated freezing and thawing.
95℃, 5 min.