Cat. No.: RNASEH
One unit refers to the amount of enzyme required to hydrolyze 1 nmol of a nucleic acid substrate from a 40 pmol RNA-DNA hybrid strand of 25 bp in length in a 50 μl reaction system at 37℃ within 20 minutes.
- 1 × Rnase H Buffer: 75 mM KCl, 50 mM Tris-HCl pH 8.3, 3 mM MgCl2, 10mM DTT.
- The optimal reaction temperature of the enzyme is 37 ° C, and the enzyme is inactivated at 65 ° C for 20min.
Store the components at -20℃. They are stable for three years under suggested storage condition. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes