Cat. No.: TPM-5
The 2 x Taq PCR MasterMix is an optimized ready-to-use PCR mixture of Taq DNA Polymerase, PCR buffer, MgCl2, dNTPs, and PCR dye. The 2 x Taq PCR MasterMix contains all components for PCR, except DNA template and primers. The Taq DNA has no 3’→5’exonuclease activity, so it is suitable to amplification of DNA fragment less than 5Kb, and the PCR product has an “A” base at the 3’terminal, thus it is very convenient to clone to T- vector.
After PCR, samples can be removed from the reaction and loaded directly onto agarose gel. The green dye, acting as a tracking dye, migrates at about the same rate as 3-5kb DNA fragment in 1% agarose gel.
5 x 1mL of 2 x Taq PCR MasterMix is supplied, which is suitable for 200 runs for 50µl PCR reaction and 500 runs for 20µl PCR reaction
Only for research and not intended for treatment of humans or animals