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2 × LongFast PCR MasterMix
$68.00 - $272.00
$340.00
2 × LongFast PCR MasterMix provides greater efficiency and elongation capabilities than conventional Taq DNA Polymerase. The PCR product has an “A” base at the 3’ terminal, thus it is very convenient to clone to T- vector. This mastermix contains red tracking dye, which can be directly loaded for electrophoresis without adding a loading buffer.
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All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: LFM-5 (for 5 ml)
Cat. No.: LFM-25 (for 25 ml)
Features
- Fast amplification speed: The extension rate can reach 6-12 kb/min.
- Excellent fidelity: The fidelity is more than 3 times that of Taq DNA Polymerase. For higher fidelity (>55 times of Taq), we recommend 2 × LongFastHiFi PCR MasterMix.
- Ideal for long sequences: When using complex genomic DNA as a template, the amplification products can be up to 10kb. When using simple genomic, plasmid, and phage DNA as templates, the amplification products can be up to 15kb.
- Strong amplification capacity: Ideal for some complex templates with special structure and templates with high GC content.
Quality Control
Each batch of 2 × LongFast PCR MasterMix must pass the amplification detection of the following two sizes of fragments before shipment:
- Hα1AT, 2.6 kb, extension 10 sec/kb
- ß-globin, 6 kb, extension 15 sec/kb.
Storage
The minimum shelf life is 2 years at -20°C and a month at 4°C.
Instruction: Protocol
Related: 2 × LongFastHiFi PCR MasterMix, 2 × FastHiFi PCR MasterMix
Only for research and not intended for treatment of humans or animals
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