2 × LongFastHiFi PCR MasterMix is based on engineered high fidelity enzymes with a variety of elongation factors, which greatly improves the amplification length, amplification speed, fidelity, and yield. The mastermix generates blunt-end PCR products because of the 3’ → 5’ exonuclease (proofreading) activity, which shows approximately 55-fold higher fidelity than Taq DNA polymerase. Therefore, 2 × LongFastHiFi PCR MasterMix provides greater efficiency and elongation capabilities than conventional PCR enzymes. This mastermix contains red tracking dye, which can be directly loaded for electrophoresis without adding a loading buffer.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: LFHF-5 (for 5 ml)
Cat. No.: LFHF-25 (for 25 ml)
Fast amplification speed: The extension rate can reach 3-4 kb/min, which is 6-8 times that of Pfu DNA Polymerase.
High amplification yield: Generally, the amount of PCR products is 50%-100% higher than that of traditional Pfu DNA Polymerase.
Excellent fidelity: The fidelity is more than 55 times that of Taq DNA Polymerase.
Ideal for long sequences: When using complex genomic DNA as a template, the amplification products can be up to 10kb. When using simple genomic, plasmid, and phage DNA as templates, the amplification products can be up to 15kb.
Each batch of 2 × LongFastHiFi PCR MasterMix must pass the amplification detection of the following two sizes of fragments before shipment:
Hα1AT, 2.6 kb, extension 20 sec/kb
ß-globin, 6 kb, extension 20 sec/kb.
The minimum shelf life is 2 years at -20°C and a month at 4°C.
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs. Besides, we have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology.
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A peptide library is a new technique for studying the structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.
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RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.
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CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene-editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments.
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GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of an organism, often a virus or bacteria that acts as a pathogen in blood, tissue, urine, etc. Based on our leading fluorescent quantitative PCR technique and isothermal amplification technology, we have developed solutions for various pathogens.