All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: FHF-5 (for 5 ml) 

Cat. No.: FHF-25 (for 25 ml) 

Features

• Fast amplification speed: The extension rate can reach 2-4 kb/min, which is 4-8 times that of Pfu DNA Polymerase.
• High amplification yield: Generally, the amount of PCR products is 50%-100% higher than that of traditional Pfu DNA Polymerase.
• Excellent fidelity: The fidelity is more than 55 times that of Taq DNA Polymerase. 
• Ideal for short sequences: When using complex genomic DNA as a template, the amplification products shall be no more than 3kb. When using simple genomic, plasmid, and phage DNA as templates, the amplification products shall be no more than 6kb. For long sequence, we recommend 2× LongHiFi PCR MasterMix.

Quality Control

Each batch of 2 × FastHiFi PCR MasterMix must pass the amplification detection of the following two sizes of fragments before shipment:

• Poly(A) Polymerase gene from E. coli gDNA, 1.4 kb, extension 20 sec/kb
• Hα1AT gene from Human gDNA, 2.6 kb, extension 30 sec/kb. 

Storage

The minimum shelf life is 2 years at -20°C and a month at 4°C.

Instruction: Protocol

Related: 2 × LongFast PCR MasterMix, 2 × LongFastHiFi PCR MasterMix

Only for research and not intended for treatment of humans or animals

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