2 × FastHiFi PCR MasterMix is based on engineered Pfu DNA polymerase, which enables fast PCR with an extension time of 2-4kb/min. The mastermix generates blunt-end PCR products because of the 3’ → 5’ exonuclease (proofreading) activity, which shows approximately 55-fold higher fidelity than Taq DNA polymerase. Therefore, 2 × FastHiFi PCR MasterMix provides greater efficiency and elongation capabilities than conventional PCR enzymes. This mastermix contains red tracking dye, which can be directly loaded for electrophoresis without adding a loading buffer.
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All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: FHF-5 (for 5 ml)
Cat. No.: FHF-25 (for 25 ml)
Fast amplification speed: The extension rate can reach 2-4 kb/min, which is 4-8 times that of Pfu DNA Polymerase.
High amplification yield: Generally, the amount of PCR products is 50%-100% higher than that of traditional Pfu DNA Polymerase.
Excellent fidelity: The fidelity is more than 55 times that of Taq DNA Polymerase.
Ideal for short sequences: When using complex genomic DNA as a template, the amplification products shall be no more than 3kb. When using simple genomic, plasmid, and phage DNA as templates, the amplification products shall be no more than 6kb. For long sequence, we recommend 2× LongHiFi PCR MasterMix.
Each batch of 2 × FastHiFi PCR MasterMix must pass the amplification detection of the following two sizes of fragments before shipment:
Poly(A) Polymerase gene from E. coli gDNA, 1.4 kb, extension 20 sec/kb
Hα1AT gene from Human gDNA, 2.6 kb, extension 30 sec/kb.
The minimum shelf life is 2 years at -20°C and a month at 4°C.