Oligonucleotide Conjugates

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Oligonucleotide Conjugates

Oligonucleotide conjugates are powerful molecular-engineering materials where oligonucleotides links with ligands such as peptides, antibodies, carbohydrates, or other small molecules. With the breakthroughs in linker chemistry and drug delivery technologies, these chimeric molecules offer the opportunity to deliver the oligonucleotide to specific cells and tissues and thus modulate the disease-causing targets and further treat the diseases. By conjugating oligonucleotides with various types of ligands, the pharmacokinetic properties of oligonucleotides are improved and the scope of applications in therapeutic cell targeting and protein diagnostics are expanded.

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Galnac Oligonucleotides

Asialoglycoprotein receptor (ASGPR) is an endocytic receptor specifically expressed by hepatocytes. In recent years, using the high-affinity ligand N-acetylgalactosamine (GalNAc) of ASGPR as the targeting molecule, a breakthrough has been made in the liver targeted delivery of oligonucleotide drugs (siRNA, ASO, microRNA). We can provide various oligonucleotides and GalNAc conjugates according to customer needs.

GalNAc siRNA is a single conjugate formed by carbohydrate compounds and siRNA. GalNAc is a targeted ligand of the sialic acid receptor (ASGPR). It has high affinity and rapid internalization ability with liver surface cells so that this kind of siRNA conjugate can specifically bind membrane proteins and then get into cells. It was found that when the single dose of GalNAc siRNA conjugate was 1 mg/ml, the subcutaneous injection could effectively silence specific target genes in the liver, and the silencing efficiency was higher than that of siRNA encapsulated in lipid nanoparticles. These siRNA conjugates have good application potential in the treatment of liver-related diseases involving gene overexpression.

In the early application research, researchers have applied GalNAc to the delivery of antisense oligonucleotide (ASO). It was found that the conjugation modification of GalNAc can improve the efficiency of the second generation ASO in mouse liver by 6-10 times. Compared with the previous generation of 2'- MOE modified ASO, combining GalNAc conjugation with the next generation of cEt modified ASO can improve the efficiency of ASO by 60 times.

In addition to the above siRNA and ASO, GalNAc is also applied to the delivery of microRNA antagonistic nucleic acid. GalNAc-microRNA has already been used for the treatment of hepatitis C. The core molecule of the drug is the antagonistic nucleic acid of miRNA-122. The antagonistic nucleic acid is connected with a GalNAc, which can be efficiently uptaken by hepatocytes and inhibit the expression of miRNA-122, so as to achieve a good anti-hepatitis C therapeutic effect (miRNA-122 is very important for HCV virus replication).

Peptide Oligonucleotides

Oligonucleotides can be used not only as the key raw materials of diagnostic reagents but also as gene therapy drugs. Because of its high specificity and good selectivity, it is considered to be an effective cancer treatment drug. However, after decades of research, oligonucleotides have not become a common and effective drug for cancer treatment. The main reasons are poor cell permeability, easy degradation by enzymes, off-target effect, and so on. The research of peptides is earlier than that of oligonucleotides, and the mechanism is relatively clear. It has better membrane permeability and a strong anti-degradation ability. Therefore, coupling oligonucleotides with peptides with various biological functions can not only improve the biological activity of oligonucleotides but also improve their cell permeability. We can provide conjugates of various oligonucleotides and peptides, such as peptide-siRNA, peptide-DNA, peptide-RNA, RGD-siRNA, etc.

PEG Oligonucleotides

The half-life of oligonucleotide drugs (aptamer, siRNA, Aso, microRNA) in vivo is very short. And the structure of oligonucleotide drugs is also easy to be damaged by various enzymes. Besides, the clearance by the kidney is also very fast, which is not conducive to the maintenance of a certain concentration of drugs. At the same time, oligonucleotides themselves have a strong negative charge and are not easy to get close to the same negatively charged cell membrane, so as to reduce their chances of uptake by cells and reduce the efficacy. The above points limit the clinical application of oligonucleotides. Although the modification of the oligonucleotide drug skeleton and the modification of the phosphodiester bond into thiophosphodiester bond can increase its resistance to nuclease, the in vivo half-life of oligonucleotide drugs still can not reach an ideal length of time, which limits the clinical application.

PEG modification can effectively solve the problem of such drugs. In the study of antisense oligonucleotide (ASO), it was found that the half-life of PEG-modified ASO with thiophosphate diester bond as the skeleton was prolonged, which was ten times that of ordinary ASO. At the same time, macromolecular PEG can increase the molecular weight and volume of oligonucleotide drugs, making it difficult for them to pass through the glomerular filtration membrane and reduce the excretion rate of the kidney, so as to effectively prolong the retention time of drugs in the circulatory system and improve their biological activity. In addition, PEG can also form a three-dimensional protective film in water, which is wound on the surface of oligonucleotide drugs to shield the negative charge on the surface, which is conducive to the uptake of oligonucleotide drugs by corresponding cells and improve the effect. We can provide various conjugates of oligonucleotides (PEG-Aptamer, PEG-siRNA, PEG-ASO) and PEG (mPEG, DSPE PEG, Branched PEG, Biotin PEG, FITC PEG, DBCO PEG, Azide PEG, etc.)

Radiolabelled Oligonucleotides

At present, PET with high sensitivity and high spatial resolution is a leading technology in the field of molecular imaging. PET combined with probes that can specifically bind to molecular targets can qualitatively and quantitatively study the process of tumorigenesis and development at the cellular and molecular level, with the advantages of in vivo, noninvasive, repetitive, and real-time. Aptamers have a strong affinity at low concentrations and can be chemically modified and labeled. Therefore, the use of radiolabeled high specific aptamer probes has unique advantages in tumor diagnosis.

In addition, the application of siRNA in vivo also faces some urgent problems to be solved, such as the off-target effect of siRNA in vivo treatment, non-specific transport and distribution, and the potential risk of activating the immune response. The in vivo distribution of siRNA is very important for RNAi technology to play a role. Therefore, the development of appropriate methods to evaluate the in vivo distribution of siRNA has attracted more and more attention. The methods used to evaluate the distribution in vivo include invasive measurement and noninvasive imaging. Invasive measurement refers to the evaluation of the distribution of probes by measuring the concentration of probes in isolated organs after killing experimental animals. Noninvasive imaging technology mainly includes radionuclide imaging, fluorescence imaging, and optical imaging technology. These technologies provide feasibility for noninvasive tracking of siRNA in vivo. Radionuclide tracing, as a widely used imaging technology in clinical and scientific research, has the characteristics of noninvasive, high sensitivity, and strong specificity. Compared with fluorescence and optical imaging technology, radionuclide imaging is non-toxic and safe, especially for large animals and even human body imaging. The application of radioactive tracer technology in siRNA in vivo imaging provides technical support for radionuclide therapy based on siRNA in the future. It can not only directly evaluate the therapeutic effect, but also achieve the dual therapeutic effect of RNAi gene therapy and radiotherapy, which is conducive to promoting the development of RNAi technology in vivo application. We can provide oligonucleotide conjugates with common radioactive markers (99mTc, 111In, 64Cu, 18F, etc., and chelating agents can be NOTA, DOTA, MAG, HYNIC, DTPA, TETA, etc.).

Oligonucleotide Fluorophores in the NIR

Fluorescence imaging technology is widely used in various biomedical research. Compared with the commonly used fluorescence imaging technology, the near-infrared fluorescence probe can significantly reduce the influence of light scattering and self fluorescence effect when penetrating blood and tissue, and make the detection depth deeper and the spatial resolution higher. The near-infrared fluorescence probe is hopeful to play an important role in the fields of in vivo imaging, early tumor diagnosis, and manual navigation in the future. We can provide oligonucleotides (siRNA, DNA, aptamer, ASO) labeled with near-infrared fluorescence (Cy7, Cy7.5, ICG, etc.), and can provide development and synthesis services of various near-infrared fluorescent nucleic acid probes according to the needs of customers

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Custom Oligos Synthesis

DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs. Besides, we have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology.

Custom Peptides Synthesis

Peptides are synthesized by the condensation reaction of the carboxyl group of one amino acid with the amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development, and peptide vaccine development. Each of our peptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis of high quality.

Gene Synthesis

Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.

DNA Synthesis Products

We have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology. The product portfolio includes Synthesis Columns for ABI 3900, MerMade, Dr. Oligos, OligoMaker, ASM-2000, etc., Universal Support-CPG, Standard Support-CPG, DNA Phosphoramidites, LNA Phosphoramidites, Modified Amidites & CPG, Molecular Sieves, etc.

Isothermal Amplification

At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase. Based on this special enzyme, we have developed PrimeIamp^TM lyophilized isothermal amplification microbeads series. These series are ready-to-use master mixes, which can perform isothermal amplification directly when the templates and primers are added. With freeze-drying technology, these master mixes are lyophilized into solid microbeads, which can be transported and stored at room temperature with great convenience.

GoodView™ Nucleic Acid Stain

GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and the other at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is included in New Products, Science Journal, January 11, 2019.

PCR-Related

Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, Pfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. High-quality dNTPs and NTPs (set, mix) are also supplied.

RNA-Related

Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide high-quality NTPs, RNasin (RNase inhibitor), and M-MLV reverse transcriptase to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series is designed for the isolation of viral RNA/DNA or bacterial DNA.

RNA Silencing

RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.

Nucleic Acid Purification

Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column) and magnetic beads technology (VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA/RNA from various sources quickly and reliably. The purity of DNA/RNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning, and cleavage.

Microspheres

Microspheres are small spherical beads formed by polymer polymerization through nanotechnology, which are widely used in the field of in vitro diagnosis. We are at the forefront of providing various types of microspheres with high quality and batch-to-batch consistency, which builds a solid foundation for large-scale production. Our products include magnetic microspheres, latex microspheres, dyed microspheres, fluorescent microspheres, flow cytometry microspheres, and standard microspheres.

DNA Markers

DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.

CRISPR Gene Editing

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene-editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments. We also offer the Cas13a Nuclease (Lyophilized) and Cas12a Nuclease (Lyophilized) that can be transported at room temperature, saving the high cost of dry ice transportation.

Biochemicals

Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids, and other biomacromolecules. We provide various types of high-quality biochemical reagents to meet these demands, including Proteinase K, Mutant Proteinase K, Besta™ LE Agarose, IPTG, X-Gal, DNase I (RNase Free), Enterokinase, SUMO Protease, TEV Protease, PreScission Protease (PSP), Bovine Serum Albumin, T4 DNA Ligase, etc.

Nuclease

A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously affect single- and double-stranded breaks in their target molecules. Here we offer various high-quality nucleases, including RNase A, RNase H, Thermostable RNase H, Benzonase Nuclease, Uracil N-Glycosylase, T7 Endonuclease I, T5 Exonuclease, Shrimp Alkaline Phosphatase, Terminal Transferase, etc.

Lab Supplies

We provide various high-quality common lab supplies, including ELISA Plates, Cell Culture Dishes, Cell Culture Plates, Microcentrifuge Tube, Centrifuge Bottles, Centrifuge Tubes, PCR Plates, PCR Tubes, Pipette Tips, Serological Pipettes, 96-well Deep-Well Plate, Conical Cryovials, SiMax™ Spin Column, etc, with high cost-effective ratio to make your experiments smoother!

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