Description

HEX Phosphoramidite is a fluorescent labeling reagent widely used in the synthesis of oligonucleotides. It incorporates a hexachloro-fluorescein (HEX) dye into DNA or RNA sequences, providing strong yellow-green fluorescence. This makes it particularly valuable in multiplex assays, where it is often paired with other dyes such as FAM or TAMRA to enable simultaneous detection of multiple targets.

HEX exhibits an excitation maximum around 535 nm and an emission maximum near 553–556 nm, producing a bright signal with minimal spectral overlap with fluorescein. It is typically introduced at the 5′ end of an oligonucleotide during automated DNA/RNA synthesis, though internal labeling is also possible with specialized chemistries.

Specifications

• CAS Number: 1360547-55-2

• Molecular Formula: C₄₆H₅₂Cl₆N₃O₁₀P

• Molecular Weight: 1050.62

• Appearance: Off-white solid

• Excitation Maximum (Ex): ~535 nm

• HPLC: ≥97%

• ³¹P NMR: ≥97%

• ¹H NMR: Conform

Key Features

• Strong yellow-green fluorescence with high sensitivity

• Compatible with standard phosphoramidite chemistry

• Suitable for 5′-end or internal labeling of oligonucleotides

• Widely used in qPCR probes, sequencing, and molecular diagnostics

Applications

• Real-time PCR (qPCR) probes for multiplex detection

• Fluorescence in situ hybridization (FISH)

• DNA sequencing and fragment analysis

• Molecular diagnostics and biomarker detection

• Multiplex assays requiring spectral separation from FAM

SBS Genetech offers a comprehensive range of phosphoramidites on the market. It is recognized as one of the global major leading industry players in Phosphoramidite by third-party market researchers. For more details, please visit Phosphoramidite - Global Forecast 2024-2030.

Only for research and not intended for treatment of humans or animals

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