*This tag is only available for services/products with more than 500 citations.

Description

Peptide synthesis is the synthesis process of peptides in organic chemistry. Peptides are organic compounds connected by multiple amino acids through peptide bonds. The manufacturing of peptides can be divided into two ways: chemical synthesis and biological synthesis. The chemical synthesis methods of peptides can be divided into two types: liquid-phase peptide synthesis and solid-phase peptide synthesis.

Mechanism

The principle of peptide synthesis is by the condensation of two amino acids in solution. One of the unprotected amino groups reacts with the other unprotected carboxylic acid group to form a peptide bond. The second reactive group (amine/acid) in each raw material carries a protective group.

After completing the synthesis of the first amide bond, the protective group of the amino group (carboxyl group) is removed, and the second amide bond is synthesized by reacting with the next protective amino acid, and so on to prolong the peptide chain.

Solid-phase Peptide Synthesis

Solid-phase peptide synthesis (SPPs), initiated by Robert Bruce Merrifield, has now become a recognized organic synthesis method for custom peptide manufacturing in the laboratory. Solid-phase peptide synthesis has the following advantages:

• Synthesize natural peptides that are difficult to express in bacteria
• Add unnatural amino acids
• Modify the peptide/protein skeleton
• Synthesize proteins with D configuration

The process of solid-phase peptide synthesis is as the following: 

1. Condensation
2. Washing 
3. Removing protection 
4. Washing

With almost 20 years of experience in solid-phase peptide synthesis, SBS Genetech is committed to supplying high-quality custom peptide(s) synthesis services at competitive prices and providing our customers with extensive services and products.

Long Peptide Synthesis

Long peptide (100-200 residues) synthesis has always been challenging for conventional chemical synthesis, therefore they are normally obtained by the expression of E.coli. However, obtaining long peptides by chemical synthesis will have the advantages of being faster and cheaper, and the resulting peptide by chemical synthesis is always correct. 

At SBS Genetech, we have gained extensive experience in synthesizing these challenging long peptides. We routinely synthesize long peptides successfully by various methods, with analytical HPLC chromatograms, MS data, and synthesis reports provided for validation. 

With our long peptide synthesis technology, non-genetically encoded structures, such as D-amino acids, can also be incorporated into long peptides. Long peptides obtained by chemical synthesis eliminate problems such as poor or no expression, cloning errors, tags like FLAG or 6-His, or the mistranslation of non-preferred codons in prokaryotic hosts. Artificial amino acids that have isosteric side chains can also be used to investigate the functional importance of specific residues.

Features

• High Purity: Purities from desalting to >98%
• Various Modifications: 400+ modifications are available, including Phosphorylation/Biotin-labeled peptides, Cyclic peptides, KLH/BSA Conjugation, MAP peptides, etc.
• Long Peptide Synthesis: The maximum length of peptide synthesis can reach 180 amino acids with our long peptide synthesis technology and various difficult and complex peptides can be synthesized.
• High Success Rate: The success rate exceeds 99%.
• Turn-around Time: For desalting peptides, the turnaround time is about 7 working days. For HPLC purified peptides, the turnaround time is about 15 working days. Analytical HPLC chromatograms, MS data, and synthesis reports are provided.
• Guarantee: If the synthesis fails due to our reason, no fee will be charged.

Peptide Modifications and Labels

• Other modifications and labels are available upon request.

With more than 20 years of experience in solid-phase peptide synthesis, SBS Genetech is recognized as one of the global major leading industry players in Peptide Synthesis by third-party market researchers. For more details, please visit Peptide Synthesis Market 2017-2022 International Outlook, Trends and Analysis.

Featured Publications

• Yang M, Chen J, Li X, Huang J, Wang Q, Wang S, Wei S, Qin Q. The transcription factor NFYC positively regulates expression of MHCIa in the red-spotted grouper (Epinephelus akaara). Dev Comp Immunol. 2021 Sep 29;127:104272. 
• Wu X, Li Y, Xu H, Chen Y, Mao H, Ma Q, Du Q, Gao P, Xia F. Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels. Anal Chem. 2021 Sep 28.
• Yaghoubi P, Bandehpour M, Mohebali M, Akhoundi B, Kazemi B. Designing and Evaluation of a Recombinant Multiepitope Protein by Using ELISA for Diagnosis of Leishmania infantum Infected in Dogs. Iran J Parasitol. 2021 Jul-Sep;16(3):377-385. 
• Xiao PP, Wan QQ, Liao T, Tu JY, Zhang GJ, Sun ZY. Peptide Nucleic Acid-Functionalized Nanochannel Biosensor for the Highly Sensitive Detection of Tumor Exosomal MicroRNA. Anal Chem. 2021 Aug 10;93(31):10966-10973. 
• Georgieva M, Heinonen T, Vitale A, Hargraves S, Causevic S, Pillonel T, Eberl L, Widmann C, Jacquier N. Bacterial surface properties influence the activity of the TAT-RasGAP_317-326 antimicrobial peptide. iScience. 2021 Jul 30;24(8):102923.
• Xu R, Li Y, Sui Z, Lan T, Song W, Zhang M, Zhang Y, Xing J. A C-terminal encoded peptide, ZmCEP1, is essential for kernel development in maize. J Exp Bot. 2021 Jul 28;72(15):5390-5406. doi: 10.1093/jxb/erab224. PMID: 34104938.
• Gu H, Ma K, Zhao W, Qiu L, Xu W. A general purpose MALDI matrix for the analyses of small organic, peptide and protein molecules. Analyst. 2021 Jun 14;146(12):4080-4086.
• Dong L, Tan CW, Feng PJ, Liu FB, Liu DX, Zhou JJ, Chen Y, Yang XX, Zhu YH, Zhu ZQ. Activation of TREM-1 induces endoplasmic reticulum stress through IRE-1α/XBP-1s pathway in murine macrophages. Mol Immunol. 2021 Jul;135:294-303.
• Heinonen T, Hargraves S, Georgieva M, Widmann C, Jacquier N. The antimicrobial peptide TAT-RasGAP_317-326 inhibits the formation and expansion of bacterial biofilms in vitro. J Glob Antimicrob Resist. 2021 Jun;25:227-231. 
• Wang Y, Xie X, Liu H, Liu H, Jiang H. LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure. Mediators Inflamm. 2021 May 28;2021:2327721.
• Gong W, Liang Y, Mi J, Jia Z, Xue Y, Wang J, Wang L, Zhou Y, Sun S, Wu X. Peptides-Based Vaccine MP3RT Induced Protective Immunity Against Mycobacterium Tuberculosis Infection in a Humanized Mouse Model. Front Immunol. 2021 Apr 26;12:666290.