The major advantage of LNA lies in the design options for primers and probes as the Tm can be fine-tuned according to the needs of the desired oligonucleotide. Due to the enhanced binding affinity, shorter probes can be realized and as a result binding specificity to the target DNA and RNA is increased. Hence, LNA oligonucleotides are very well suited for use in antisense protocols, hybridization assays, in situ hybridization probes, dual-labeled probes, molecular beacons, and qPCR primers.
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Increased Thermal Stability of qPCR Probes
Improved affinity and specificity
Reduced background fluorescence
Better signal-to-noise ratio
Enhanced Single Nucleotide Discrimination
Greatly enhanced discrimination between alleles via single nucleotide polymorphism (SNP)
Better mismatch discrimination compared to native-state DNA probes
Multiplex qPCR Systems
Normalization of the Tm across several short sequences with varying GC-content gets accessible
Optimal design of highly-specific, shorter probes
Particularly beneficial for microarray and multiplex PCR applications
Enhanced binding affinity to complementary nucleic acids
High nuclease resistance
Further increase in nuclease resistance by the introduction of a phosphorothioate backbone
* The price of custom LNA oligo is dependent on the length, yield, and number. Please indicate the specifics in the quote request.
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Long non‐coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution
The locked nucleic acids (LNA) targeting ZIKV sfRNA were chemically synthesized and HPLC purified by Beijing SBS Genetech Co., Ltd. For preparation of ZIKV sfRNA-LNA complex sample, ZIKV sfRNA was mixed with LNA in a 1:10 molar ratio and incubated overnight at 4°C, further purified with SEC column, and then exchanged into SAXS buffer as above.
Only for research and not intended for treatment of humans or animals