
CpG ODN Synthesis Service
Description
CPG ODN (CpG oligonucleotide) is a synthetic oligodeoxynucleotide containing unmethylated cytosine-phosphate-guanine (CpG) motifs. It mimics bacterial DNA and stimulates the immune cells of various mammals, including humans. CPG ODN can directly activate B cells and monocytes (macrophages and dendritic cells), as well as indirectly activate NK cells and T cells, among other immune effector cells. It enhances their function and cytokine secretion, improves antigen processing and presentation, induces a Th1-type immune response, and elicits robust humoral and cellular immune responses, enhancing both specific and nonspecific immune reactions.
The mammalian innate immune system recognizes pathogenic microorganisms through pattern recognition receptors, and Toll-like receptor 9 (TLR9) is one of the key innate immune pattern recognition receptors. The natural ligand for TLR9 is unmethylated cytosine-phosphate-guanine (CpG) sequences found in viral and bacterial genomes. In experimental studies, synthetic oligodeoxynucleotides containing CpG motifs, which are resistant to nucleases, are commonly used as TLR9 ligand analogs. Activation of TLR9 by its ligand leads to a strong Th1-dominant immune response, making it promising for the treatment of allergic diseases, infectious diseases, and cancer.
CpG ODN induces the production of cytokines and activation of various immune cells, exhibiting anti-tumor effects. CpG ODN can exert its anti-tumor effects through multiple pathways, including being used as a standalone therapeutic agent, an immune adjuvant, or in combination with other immunotherapies. It can also be used in conjunction with radiotherapy, chemotherapy, and surgery for tumor treatment. The anti-tumor activity of CpG ODN has been demonstrated in animal models, and new anti-tumor drugs based on the characteristics of CpG ODN are being developed. Several CpG ODN-based drugs have entered clinical trial stages, offering new approaches for the treatment of cancer patients.
Service Details
Different types of CpG ODN have distinct structural features and immunological effects. They are generally classified into three types: A, B, and C. We can provide multiple CpG ODN variants belonging to these three types.
Type A CpG ODNs have a palindromic sequence containing CpG dinucleotides as the core, with poly-G tails at both ends. They have a phosphorothioate backbone partially modified with sulfur. The palindromic sequence and poly-G form higher-order structures. Type A CpG ODNs can activate plasmacytoid dendritic cells to induce a large amount of type I interferon production but have weaker activity on B cells.
Type B CpG ODNs are fully sulfur-modified linear CpG ODNs. They exhibit strong immune-stimulatory activity on B cells but do not activate plasmacytoid dendritic cells.
Type C CpG ODNs are fully sulfur-modified CpG ODNs that can form dimers through the palindromic sequence. They possess the combined activity of both type A and type B CpG ODNs, activating both plasmacytoid dendritic cells and B cells.
Related: Probes for Quantitative, Real-time PCR, Molecular Beacons, Oligonucleotide Conjugates

Selected Published Papers
SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood
Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level
Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100
All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).
Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant
Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828
To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)
A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes
Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543
HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier
Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation
Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055
Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).
Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection
Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089
Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......
Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor
Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019
Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC
Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions
Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014
Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).
A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin
International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211
The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.
Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer
Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465
The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.
Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva
Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015
The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...
Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection
Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318
Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.