16S/18S FISH Probe Design & Synthesis
Description
The 16S and 18S rRNA sequences are the preferred targets for members of the bacterial/archaeal and eukaryotic domains because they are universally present and highly abundant, consisting of highly conserved regions and variable regions. The FISH technique utilizes these gene sequences to design specific probes that can accurately locate and identify target microorganisms. This provides a powerful tool for analyzing microbial community structures, studying ecological functions, and diagnosing diseases. Probe design is indeed a critical step that can affect the performance of FISH. After years of development, we have launched a species-specific 16S/18S FISH probe design, synthesis, and detection service, offering researchers an unparalleled 16S/18S FISH probe experimental experience.
Selection of Fluorescent Labels for FISH Probes
The table below lists the wavelength ranges of the filter sets for the four commonly used fluorescent channels in fluorescence microscopy: DAPI, FITC, Texas Red, and CY5. It also includes the types of fluorescent groups that are most compatible with these channels, along with their primary applications (such as antibody labeling, protein expression, or FISH probe labeling):
| Filter Set | Excitation Filter (nm) | Emission Filter (nm) | Best Matching Fluorophore Types | Compatible Fluorophore Types | Main Applications |
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Please note that these values and the recommended fluorophores are based on common fluorescence microscope filter settings and the characteristics of fluorescent dyes. In practical applications, it is advisable to refer to the microscope’s user manual, the specifications from filter manufacturers, and information from fluorescent dye suppliers to ensure optimal fluorescence signal and imaging results.
Recommended FISH Probe Fluorescent Labeling Strategies (Please confirm based on actual conditions):
Single Bacterium 16S FISH Labeling:
- Target Bacterium Labeling: Preferably label with Texas Red, compatible with CY3
- Negative Control: Recommended to label with FITC (*Microbial FISH services can provide this for free)
- Positive Control (Universal Bacterial Probe): CY5
- DAPI Channel: Stains DNA
Dual-Color FISH Probe Labeling (Simultaneously detecting two bacteria, or using two different fluorescent labels to detect the same bacterium):
- Positive Control (Universal Bacterial Probe): FITC
- Target Bacterium 1: Recommended to label with Texas Red, compatible with CY3
- Target Bacterium 2: Recommended to label with CY5
- DAPI Channel: Stains DNA
In this case, if a negative control is needed, it should be tested on a separate slide.
Product Features
- Each probe is rigorously designed and evaluated to ensure high specificity and high coverage.
- Each probe comes with a detailed evaluation report, including specificity, coverage, and application recommendations.
- For single FISH probes with poor specificity, dual probes and multiple probes are designed to improve specificity.
Benefits of Using Dual Probes in FISH 16S rRNA Detection:
- Increased Specificity: Since the 16S rRNA gene has different conserved and variable regions in different bacteria, using dual probes can simultaneously target these regions, thereby enhancing the specific identification of the target species.
- Improved Sensitivity and Accuracy: Dual probes provide double confirmation, reducing false positives caused by potential non-specific binding of a single probe.
- Wide Applicability: Dual-probe FISH technology can be applied to the detection of various microorganisms, including bacteria, archaea, and fungi, making it a versatile tool for microbial detection.
- Aids in Microbial Community Analysis: In microbial community structure analysis, dual probes can more accurately identify and distinguish different members of the community, providing important information for microbial ecology research.
With over 20 years of leading experience in oligo synthesis, SBS Genetech is recognized as one of the global major leading industry players in Oligonucleotide Synthesis by third-party market researchers. For more details, please visit Oligonucleotide Synthesis Services Market landscape, Top Competitor Analysis, Revenue, Sales With Forecast Data from 2022 to 2028.
Selected Published Papers
SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood
Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level
Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100
All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).
Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant
Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828
To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)
A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes
Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543
HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier
Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation
Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055
Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).
Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection
Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089
Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......
Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor
Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019
Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC
Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions
Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014
Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).
A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin
International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211
The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.
Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer
Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465
The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.
Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva
Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015
The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...
Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection
Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318
Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.
