Description

By integrating ONT's (Oxford Nanopore Technologies) long-read real-time sequencing with 10X Genomics technology, the full-length cDNA generated through reverse transcription can be directly sequenced and rapidly analyzed. This approach allows for the effective acquisition of high-quality, full-length RNA sequences from individual molecules. It enables precise identification of isoforms, homologous genes, superfamily genes, or allele-specific transcripts that second-generation sequencing technologies often fail to distinguish.

Technical Advantages

• High Cell Capture Throughput: The technique allows for the simultaneous analysis of a large number of cells, greatly enhancing throughput.
• Long Read Lengths: The use of long-read sequencing technology captures extensive sequences in a single read.
• High Number of Reads: Generates a substantial number of reads, increasing the depth of analysis.
• High Multiplexing Capability: A single chip can process tens of thousands of cells, facilitating large-scale studies.

Experimental Principle

Nanopore sequencing detects signals by monitoring the potential difference across a nanopore as molecules pass through it. The four nucleotide bases (A, T, C, and G) have distinct electrical properties, which cause unique variations in the electrical signal. By analyzing these differences, the specific base passing through the nanopore can be identified, thus enabling accurate sequencing.