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Cat. No.: XHOIEK-96 (for 96T)

Cat. No.: XHOIEK-480 (for 480T)

Description

This product uses a sandwich ELISA method to detect the content of XhoI in samples. XhoI-specific antibodies are pre-coated on the microplate. When used, standard samples and test samples are added separately to the reaction wells already coated with specific antibodies. XhoI in the samples will bind to the antibodies in the microplate. After incubation, washing is performed to remove unbound substances inside the microplate. Then, XhoI detection antibodies (HRP labeled) are added to form a complex of antibody-antigen-HRP labeled antibody. TMB is added for color development, and the intensity of color development is proportional to the concentration of XhoI. The XhoI residue in the samples is determined by measuring the color development of XhoI standards.

• Detection range: 0.125~4 ng/ml
• Quantification limit: 0.125 ng/ml
• Detection limit: 0.021 ng/ml

Storage

Store at 2-8°C. The kit can be stored at 2-8°C in an unopened state for 12 months. Avoid exposure to light.

Precautions

• This product is for research use only and is not intended for clinical diagnosis or treatment.
• Reagents should be stored according to the conditions indicated on the label, and both reagents and samples should be equilibrated to room temperature before use.
• Prior to use, the pre-coated enzyme plate should be equilibrated to room temperature, and the remaining strips should be immediately returned to the packaging and resealed for storage at 2-8°C. Any remaining reagents should also be resealed and stored according to the conditions specified on the label.
• XhoI standard and XhoI detection antibody (HRP labeled) solutions should be mixed well before use.
• Liquid remaining in the wells during washing should be tapped dry on clean, dust-free paper. Overdrying of the wells after tapping should be avoided, as improper washing can result in higher CV values for duplicate wells or higher blank (0 ng/ml) absorbance values, affecting result accuracy.
• The TMB substrate should be a colorless, transparent liquid. If it appears light blue, it indicates contamination and is unsuitable for subsequent analysis.
• Samples with extreme pH values (<5.0 or >8.5), or containing high salt, high polysaccharide, high urea, high organic solvent, or high detergent solutions, may lead to lower recovery rates.
• Due to the complexity of biological sample matrices, it is recommended to include positive control samples in each assay to ensure accuracy, and to use duplicate wells for testing.

Safety Precautions

• The terminating solution is an acidic solution, so safety precautions should be taken when using it.
• Operators should wear personal protective equipment such as lab coats, gloves, masks, and goggles.

Related:

• Benzonase ELISA Kit
• BsaI ELISA Kit
• XhoI ELISA Kit
• XbaI ELISA Kit
• BsmBI ELISA Kit

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory