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Cat. No.: UNG-5k (for 5,000U)

Cat. No.: UNG-50k (for 50,000U)

For the heat-labile version, please visit HL-UDG.

Description

UNG is mainly used to prevent contamination of PCR amplification products. The principle is: if dUTP is incorporated into DNA instead of dTTP in the PCR reaction, a dU-containing PCR amplification product is formed. The enzyme can selectively break the glycosidic bonds of U groups in single-stranded and double-stranded DNA, and degrade the PCR amplification product.

Application

• Removal of uracil bases from single- or double-stranded DNA
• Removal of residual contamination from PCR

Unit Definition

Under a standard PCR reaction system, the amount of enzyme degrading 1 g dU-containing single-stranded DNA in 1 hour at 37°C is one active unit.

Storage

Stored at -20°C for 2 years. Avoid repeated freezing and thawing.

Thermal Inactivation

95°C, 5 min.

Related:

• Uracil N-Glycosylase
• Heat-Labile Uracil DNA Glycosylase (HL-UDG)
• Heat-Labile Uracil DNA Glycosylase 2.0 (HL-UDG 2.0)
• Thermostable UDG (Uracil-DNA Glycosylase)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory