Cat. No.: UNG-1k (for 1,000U)
Cat. No.: UNG-5k (for 5,000U)
Description
UNG is mainly used to prevent contamination of PCR amplification products. The principle is: if dUTP is incorporated into DNA instead of dTTP in the PCR reaction, a dU-containing PCR amplification product is formed. The enzyme can selectively break the glycosidic bonds of U groups in single-stranded and double-stranded DNA, and degrade the PCR amplification product.
Application
Unit Definition
Under standard PCR reaction system, the amount of enzyme degrading 1 g dU-containing single-stranded DNA in 1 hour at 37℃ is one active unit.
Reaction Buffer
Uracil DNA Glycosylase (UNG) is compatible with most PCR polymerase reaction buffers, but its activity is inhibited at high ion concentrations (>100 mM).
Preservation Solution
20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 50% Glycerol, 0.1% (w/v) Triton X-100, pH 7.5.
Storage
Stored at -20 C for 2 years. Avoid repeated freezing and thawing.
Thermal Inactivation
95℃, 5 min.
Only for research and not intended for treatment of humans or animals
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