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Cat. No.: TTHDL-2k (for 2KU)

Cat. No.: TTHDL-10k (for 10KU)

Description

Tth DNA Ligase catalyzes the formation of a phosphodiester bond between the 5’-phosphate and 3’-hydroxyl groups in double-stranded DNA, using NAD+ as a coenzyme and energy source for the reaction. Ligation occurs only when the oligonucleotide is perfectly paired with the complementary target DNA and there are no gaps between them. It is inactive on single-stranded DNA or RNA and blunt-ended DNA. Therefore, it can detect single base mutations. Its high thermal stability allows ligation under high-density hybridization conditions, enabling precise detection of SNPs with high specificity and stringency.

Our Tth DNA Ligase is a recombinant protein expressed and purified through multiple steps.

Activity Definition

One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 μg of BstEII-digested λ DNA fragments (12 bp sticky ends, equivalent to 0.0338 pmole) in a 20 μl reaction system at 45°C within 15 minutes.

Activity Assay Conditions

• 20 mM Tris-HCl (pH 7.6)
• 25 mM Potassium Acetate
• 10 mM Magnesium Acetate
• 1 mM NAD+
• 10 mM DTT
• 0.1% TritonX-10
• Reaction at 45°C

Features

• High thermal stability
• Capable of ligating nicks in DNA strands under high-temperature conditions

Applications

• Ligation of phosphorylated oligonucleotides in ligase chain reaction (LCR)
• Allele-specific detection in ligase chain reaction

Storage 

Store at -20°C.

DNA Ligase Products:

• T4 DNA Ligase
• T4 DNA Ligase (glycerol-free)
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory