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Cat. No.: T4DL-40k (for 40KU)

Cat. No.: T4DL-200k (for 200KU)

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl termini of double-stranded DNA or RNA with sticky or blunt ends. ATP is required as a cofactor for this reaction. In addition, T4 DNA Ligase is capable of repairing single-strand nicks in double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids.

Applications

T4 DNA Ligase is commonly used for ligation between DNA fragments and vectors, linkers or adaptors. It can also be applied in nick repair and ligase-mediated RNA detection.

Source

This T4 DNA Ligase is recombinantly expressed in Escherichia coli, with the encoding gene derived from bacteriophage T4.

Unit Definition

One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C.

Storage

Store at -20°C.

Precautions

• For routine chemical transformation of E. coli, purification of ligation products is not required; the ligation mixture can be used directly for transformation. However, for electrotransformation of E. coli, it is generally recommended to purify the DNA via DNA purification kits or phenol-chloroform extraction prior to electroporation.
• Gel electrophoresis analysis is unnecessary for standard ligation reactions. If electrophoresis of ligation products is required, incubate the sample at 65°C for 10 minutes to inactivate T4 DNA Ligase beforehand. This prevents band shift caused by binding between T4 DNA Ligase and DNA.
• This product is intended solely for scientific research use by trained professionals. It must NOT be used for clinical diagnosis or therapy, food processing or pharmaceutical manufacturing. Do not store this product in residential premises.
• For your personal safety and health, always wear a lab coat and disposable gloves during handling.

DNA Ligase Products:

• T4 DNA Ligase
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032

Only for research and not intended for treatment of humans or animals

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