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Cat. No.: T4DL-40k (for 40KU)

Cat. No.: T4DL-200k (for 200KU)

Components

Unit Definition

One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C.

Protocol

1. Set up the following reaction in a microcentrifuge tube on ice

2. For cohesive (sticky) ends, incubate at 16°C for 3 hours or 4°C overnight.

Storage

The minimum shelf life is 1 year at -20°C and 3 years at -80°C.

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032

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