Terminal Transferase (20 U/μl)
$135.00 - $500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: TT-500 (for 500U)
Cat. No.: TT-2500 (for 2,500U)
Description
This terminal transferase (TdT) has a molecular weight of 58.3 kDa and no 5' and 3' exonuclease activities. The addition of Co2+ to the reaction can improve the tailing efficiency. The enzyme is widely used in DNA 3' end addition homopolymer, DNA 3' end labeling using modified bases (such as ddNTP, DIG-dUTP), TdT-mediated dUTP nick end labeling technology (in situ detection of apoptosis), and other tests. The enzyme is tested free of DNA endonuclease and exonuclease contamination and RNase.
Application
- Oligonucleotide or DNA 3' hydroxyl end labeling
- DNA tailing
- 5'-RACE
- Synthesis of oligomeric strands of the same deoxynucleotide
Unit Definition
The amount of enzyme required to catalyze the addition of 1 nmol dNTP to the 3' hydroxyl end of a polynucleotide within 60 min at 37°C is defined as an active unit.
1 x TdT Buffer
100 mM KCl, 30 mM Tris-acera, 0.05% (v/v) Triton X-100 (pH 7.5 at 25°C)
Storage
Stored at -20°C for 3 years.
Thermal inactivation
75°C for 20 min.
Notes
- The activity of Terminal Transferase can be lost by an appropriate amount of EDTA.
- Metal ion chelators, higher concentrations of ammonium ions, chloride ions, iodine ions, and phosphate ions all have inhibitory effects on Terminal Transferase activity.
