Cat. No.: TT-500 (for 500U)
Cat. No.: TT-2500 (for 2,500U)
Description
This terminal transferase (TdT) has a molecular weight of 58.3 kDa and no 5' and 3' exonuclease activities. The addition of Co2+ to the reaction can improve the tailing efficiency. The enzyme is widely used in DNA 3' end addition homopolymer, DNA 3' end labeling using modified bases (such as ddNTP, DIGdUTP), TdT-mediated dUTP nick end labeling technology (in situ detection of apoptosis) and other tests. The enzyme is tested free of DNA endonuclease and exonuclease contamination and RNase.
Application
Unit Definition
The amount of enzyme required to catalyze the addition of 1 nmol dNTP to the 3' hydroxyl end of a polynucleotide within 60 min at 37℃ is defined as an active unit.
1 x TdT Buffer
100 mM KCl, 30 mM Tris-acera, 0.05% (v/v) Triton X-100 (pH 7.5 at 25℃)
Storage
Stored at -20℃ for 3 years.
Thermal inactivation
75℃ for 20 min.
Notes