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Cat. No.: T7EX-1k (for 1000U)

Cat. No.: T7EX-10k (for 10KU)

Description

T7 Exonuclease acts on double-stranded DNA and catalyzes the removal of 5' single nucleotide along the 5' → 3 'direction. It can start digestion from the 5' end, as well as from the cut or notch of double-stranded DNA. It can degrade both 5' phosphorylated DNA and 5' dephosphorylated DNA. It is reported that it can degrade RNA or DNA on the double-stranded RNA/DNA hybridization along the 5 '→ 3' direction, but cannot degrade double-stranded or single-stranded RNA.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the production of 1 nmol acid-soluble deoxyribonucleotide from double-stranded DNA substrate within 30 minutes in a 50 μl reaction system at 25°C.

Components

T7 Exonuclease (10 U/μl):    100 μl/1 ml
10×T7 Exo Buffer:    1 ml/1 ml×5

Storage

The minimum shelf life is 3 years at -20°C.

Note

• 1×T7 Exo Buffer: 50 mM KAc, 20 mM Tris-Ac, 10 mM Mg(Ac)2, 1 mM DTT (pH 7.9 @ 25°C), 25°C incubation.

• The optimal reaction temperature of the enzyme is 25°C, and it can be inactivated at 75°C for 20 minutes.