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Cat. No.: T4G46L-100 (for 100 μg)

Cat. No.: T4G46L-500 (for 500 μg)

Description

T4 phage replication can be divided into two types: initiator-dependent replication and recombinase-dependent replication. The 3'- ssDNA generated from initial replication is used as the first step of strand invasion in recombination replication. The 3' - ssDNA is covered by T4 gp32 protein, and T4 UvsY is recruited here at the same time. T4 UvsY is carried here as the loader of T4 UvsX. At the same time, gp32 is replaced. UvsX and UvsY form a synaptic structure and then invade homologous double strands to form D-Loop. Once D-Loop is formed, UvsX is replaced. D-loop becomes the template of the lagging strand and is quickly combined with gp32. Subsequently, the helicase loader gp59 combines with the DNA replication fork covered by gp32 and carries gp41/61 here. The gp61 primase can synthesize primer fragments to promote the synthesis of DNA on the lagging strand, while gp41 promotes the DNA synthesis of the leading strand by unwinding the template. Finally, the slide protein gp45 and its loader protein gp44/62 of the polymerase combine with the lead strand to promote the assembly of the polymerase gp43. After gp45 carries gp43 to the replication fork, it starts the synthesis of the lead strand and the lag strand, and then gp44/62 is dissociated from the replication fork. The gp61 primase can synthesize oligonucleotides to trigger the synthesis of Okazaki fragments on the lagging strand. Therefore, gp44/62, as the loader of gp45, plays an important role in DNA synthesis guided by polymerase gp43.

Components

100 μg

T4 GP44/62 Clamp Loader (1 μg/μl):    100 μl

Storage

The minimum shelf life is 3 years at -20°C.

Note

• Storage Buffer: 200mM Tris-HCl (pH7.5), 200mM NaCl, 5mM DTT, 25% glycerol.