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Cat. No.: RFTO-50 (for 50μg)

Cat. No.: RFTO-200 (for 200μg)

Description    

Recombinant Human FTO Protein (EC 1.14.11.53), also known as Fat Mass and Obesity-associated Protein (FTO), is an RNA demethylase. FTO belongs to the family of α-ketoglutarate (α-KG) and Fe(II)-dependent dioxygenases, and it can efficiently remove N6-methyladenosine (m6A) modifications from single-stranded RNA (ssRNA). In addition, it catalyzes the oxidative demethylation of 3-methyluridine (m3U) in ssRNA and 3-methylthymidine (m3T) in ssDNA. FTO shows a preference for single-stranded nucleic acids, favoring ssDNA/RNA as substrates, with methylated RNA as its primary substrate.

The reaction principle of FTO-mediated m6A demethylation in ssDNA/RNA is as follows: In vitro, FTO catalyzes m6A demethylation in an α-ketoglutarate and Fe(II)-dependent manner. During the reaction, α-ketoglutarate is first converted into succinate and CO₂, generating an unstable hydroxymethyl intermediate. This hydroxymethyl group then decomposes into formaldehyde and a demethylated base, which is subsequently converted into adenosine.

The FTO gene is found only in vertebrates and algae, and in vertebrates it is highly expressed in the hypothalamus, pituitary gland, and adrenal glands. The FTO protein is localized in the cell nucleus.

FTO is closely associated with a range of metabolic and neurodegenerative diseases, including obesity, diabetes, non-alcoholic steatohepatitis (NASH), and Alzheimer’s disease. Single-nucleotide polymorphisms (SNPs) within the FTO gene are linked not only to obesity but also to several cancers, such as endometrial cancer, breast cancer, pancreatic cancer, and melanoma.

In addition, FTO plays a role in regulating adipogenesis. Dysregulation of FTO and m6A RNA modification has been implicated in multiple cancers, including acute myeloid leukemia, glioblastoma, cervical squamous cell carcinoma, and melanoma. Consequently, FTO has emerged as a potential therapeutic target in precision oncology.

Components

• Recombinant Human FTO Protein (1mg/ml)
• Reaction Buffer I (10X)
• Reaction Buffer II (10X)

Applications

Suitable for FTO enzyme kinetics, inhibitor screening, and selectivity analysis.

Source

Human FTO gene expressed in E. coli.

Purity

Free of DNases (endonucleases and exonucleases), RNases, and phosphatases.

Enzyme Storage Buffer

• 150 mM NaCl
• 50% (v/v) Glycerol
• 50 mM HEPES (pH 8.0 @ 25 °C)

Inactivation / Inhibition

Add 5 mM EDTA, followed by heating at 95 °C for 5 minutes.

Storage

Store at –20 °C. Stable for at least one year under recommended conditions.

Notes

• During use, keep the product on ice packs or in an ice bath. Return immediately to –20 °C after use.
• As this product involves RNA and DNA handling, follow strict RNA/DNA operation guidelines to avoid RNase and DNase contamination.
• Laboratory consumables such as pipette tips and centrifuge tubes must be RNase- and DNase-free, or pre-treated with DEPC.
• Depending on the application, additional reagents may be required, such as RNase inhibitors or ultrapure water.
• For research use only. Not intended for clinical diagnosis or therapy, not for use in food or pharmaceuticals, and not to be stored in residential settings.
• For your safety and health, always wear a lab coat and disposable gloves when handling this product.

Only for research and not intended for treatment of humans or animals

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