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Prime T7 RNA Polymerase

Prime T7 RNA Polymerase

$500.00 - $4,000.00
Prime T7 RNA polymerase has high specificity for T7 phage promoters and can use it as a promoter and DNA as a template for the synthesis of sense-stranded RNA in vitro. Both linear plasmids and PCR product double-stranded DNA can be used as substrate templates for Prime T7 RNA polymerase. Prime T7 RNA polymerase has been engineered by electronic reconstruction and library screening. Compared to the wild type, the enzyme has a higher affinity for the DNA template binding region, enabling it to have a sustained synthesis ability, resulting in higher yields and easier synthesis of long fragments of RNA. The enzyme is a recombinant purified product without DNase and RNase contamination.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: PT7P-5k (for 5KU)

Cat. No.: PT7P-25k (for 25KU)

Cat. No.: PT7P-200k (for 200KU)

 

For the standard version, please visit T7 RNA Polymerase.

 

Description

Prime T7 RNA Polymerase exhibits high specificity for the T7 bacteriophage promoter, and uses this promoter together with a DNA template to synthesize sense‑strand RNA in vitro. Both double‑stranded linearized plasmid DNA and PCR products can serve as suitable templates for this enzyme.

Compared with the wild‑type enzyme, Prime T7 RNA Polymerase—engineered through structural reconstruction and library screening—shows significant improvements in several key performance attributes:

  • Higher DNA‑binding affinity in the template‑interaction region, enabling more processive RNA synthesis. This results in higher overall yield and facilitates efficient transcription of long RNA fragments.
  • Substantially enhanced thermostability, allowing robust activity across 37–45 °C. Elevated‑temperature transcription reduces dsRNA secondary structures, which is advantageous for the preparation of therapeutic RNAs.

The enzyme is a recombinant, highly purified product, free of DNase and RNase contamination.

 

Applications

  • Radiolabeled RNA probe preparation
  • RNA generation for studies of the RNA structure, processing, and catalysis
  • Expression control via anti-sense RNA
  • mRNA, sgRNA synthesis

 

Storage

The minimum shelf life is 2 years at - 20°C. If there are white precipitates, please use the enzyme after a warm bath at 37°C for 5 minutes. This will not affect the performance.

 

Heat inactivation

75°C，10min

 

Instruction: Protocol

 

Related: HY High-Yield T7 RNA Synthesis Kit, RNasin (RNase inhibitor), NTP, DNase I (RNase Free), Thermostable Inorganic Pyrophosphatase

 

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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